Gap Junctional Communication Modulates Gene Transcription by Altering the Recruitment of Sp1 and Sp3 to Connexin-response Elements in Osteoblast Promoters

Joseph P. Stains, Fernando Lecanda, Joanne Screen, Dwight A. Towler, Roberto Civitelli

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Loss-of-function mutations of gap junction proteins, connexins, represent a mechanism of disease in a variety of tissues. We have shown that recessive (gene deletion) or dominant (connexin45 overexpression) disruption of connexin43 function results in osteoblast dysfunction and abnormal expression of osteoblast genes, including down-regulation of osteocalcin transcription. To elucidate the molecular mechanisms of gap junction-sensitive transcriptional regulation, we systematically analyzed the rat osteocalcin promoter for sensitivity to gap junctional intercellular communication. We identified an Sp1/Sp3 containing complex that assembles on a minimal element in the -70 to -57 region of the osteocalcin promoter in a gap junction-dependent manner. This CT-rich connexin-response element is necessary and sufficient to confer gap junction sensitivity to the osteocalcin proximal promoter. Repression of osteocalcin transcription occurs as a result of displacement of the stimulatory Sp1 by the inhibitory Sp3 on the promoter when gap junctional communication is perturbed. Modulation of Sp1/Sp3 recruitment also occurs on the collagen Iα1 promoter and translates into gap junction-sensitive transcriptional control of collagen Iα1 gene expression. Thus, regulation of Sp1/Sp3 recruitment to the promoter may represent a potential general mechanism for transcriptional control of target genes by signals passing through gap junctions.

Original languageEnglish (US)
Pages (from-to)24377-24387
Number of pages11
JournalJournal of Biological Chemistry
Volume278
Issue number27
DOIs
StatePublished - Jul 4 2003

Fingerprint

Connexins
Gap Junctions
Osteocalcin
Osteoblasts
Response Elements
Transcription
Genes
Communication
Collagen
Recessive Genes
Gene Expression
Connexin 43
Gene Deletion
Genetic Promoter Regions
Gene expression
Rats
Down-Regulation
Modulation
Tissue
Mutation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Gap Junctional Communication Modulates Gene Transcription by Altering the Recruitment of Sp1 and Sp3 to Connexin-response Elements in Osteoblast Promoters. / Stains, Joseph P.; Lecanda, Fernando; Screen, Joanne; Towler, Dwight A.; Civitelli, Roberto.

In: Journal of Biological Chemistry, Vol. 278, No. 27, 04.07.2003, p. 24377-24387.

Research output: Contribution to journalArticle

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