Gastric A cell function in insulin deprived depancreatized dogs

E. Blazquez, L. Munoz Barragan, G. S. Patton, L. Muñoz-Barragan, L. Orci, Roger H Unger, R. H. Unger

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

To determine if gastric A cells are a major source of the glucagonemia of insulin deprived depancreatized dogs and to examine their secretory behavior, immunoreactive glucagon (IRG) was measured simultaneously in plasma from the inferior vena cava (VC) and from a gastric vein (GV) draining the fundus. Basal GV IRG averaged 205±35 pg/ml, significantly above the VC level of 71±30 (P<0.001) and rose to 1417±498 1.5 minutes after the start of an arginine infusion, exceeding VC IRG at all points (P<0.01). Measurement of IRG in gastric, jejunal, and ileal veins and vena cava revealed an IRG gradient only across the stomach. Measurement of glucagon like immunoreactivity (GLI) revealed no gradient across the stomach, jejunum, or ileum, thus excluding cross reaction with GLI as the cause of the GV hyperglucagonemia. Intragastric arginine elicited a near doubling of GV IRG within 1.5 minutes and this persisted for at least 120 minutes, ranging from 142 to 623 pg/ml above the VC level. Infusion of insulin at a physiologic rate lowered GVIRG from 665±66 to 151±49 pg/ml in 20 minutes and abolished the GV VC gradient within 60 minutes, whereas intravenous and intragastric glucose administration without insulin did not alter GV IRG. It is concluded that: 1) in the insulin deprived depancreatized dog, the stomach is a major source of IRG; 2) gastric IRG secretion is somehow stimulated by intravenous and intragastric arginine administration; 3) it is not influenced by intravenous or intragastric glucose administration; and 4) its release is suppressed by physiologic levels of insulin.

Original languageEnglish (US)
Pages (from-to)1182-1188
Number of pages7
JournalEndocrinology
Volume99
Issue number5
StatePublished - 1976

Fingerprint

Stomach
Glucagon
Dogs
Insulin
Venae Cavae
Veins
Arginine
Cerebral Veins
Glucose
Cross Reactions
Inferior Vena Cava
Jejunum
Ileum

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Blazquez, E., Munoz Barragan, L., Patton, G. S., Muñoz-Barragan, L., Orci, L., Unger, R. H., & Unger, R. H. (1976). Gastric A cell function in insulin deprived depancreatized dogs. Endocrinology, 99(5), 1182-1188.

Gastric A cell function in insulin deprived depancreatized dogs. / Blazquez, E.; Munoz Barragan, L.; Patton, G. S.; Muñoz-Barragan, L.; Orci, L.; Unger, Roger H; Unger, R. H.

In: Endocrinology, Vol. 99, No. 5, 1976, p. 1182-1188.

Research output: Contribution to journalArticle

Blazquez, E, Munoz Barragan, L, Patton, GS, Muñoz-Barragan, L, Orci, L, Unger, RH & Unger, RH 1976, 'Gastric A cell function in insulin deprived depancreatized dogs', Endocrinology, vol. 99, no. 5, pp. 1182-1188.
Blazquez E, Munoz Barragan L, Patton GS, Muñoz-Barragan L, Orci L, Unger RH et al. Gastric A cell function in insulin deprived depancreatized dogs. Endocrinology. 1976;99(5):1182-1188.
Blazquez, E. ; Munoz Barragan, L. ; Patton, G. S. ; Muñoz-Barragan, L. ; Orci, L. ; Unger, Roger H ; Unger, R. H. / Gastric A cell function in insulin deprived depancreatized dogs. In: Endocrinology. 1976 ; Vol. 99, No. 5. pp. 1182-1188.
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