Gata2 is a rheostat for mesenchymal stem cell fate in male mice

Xiaoxiao Li, Hoangdinh Huynh, Hao Zuo, Marjo Salminen, Yihong Wan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Gata2 is a zinc finger transcription factor that is important in hematopoiesis and neuronal development. However, the roles of Gata2 in the mesenchymal lineages are poorly understood. In vitro studies suggest that Gata2 modulates adipocyte differentiation and mesenchymal stem cell (MSC) proliferation. To systematically determine the in vivo functions of Gata2 in the MSC lineage commitment and development, we have generated three mouse models in which Gata2 is specifically deleted in MSCs, adipocytes, or osteoblasts. During the MSC expansion stage, Gata2 promotes proliferation and attenuates differentiation; thereby Gata2 loss in MSCs results in enhanced differentiation of both adipocytes and osteoblasts. During the differentiation stage, Gata2 also plays MSC-independent roles to impede lineage commitment; hence, Gata2 loss in adipocyte or osteoblast lineages also augments adipogenesis and osteoblastogenesis, respectively. These findings reveal Gata2 as a crucial rheostat of MSC fate to control osteoblast and adipocyte lineage development.

Original languageEnglish (US)
Pages (from-to)1021-1028
Number of pages8
JournalEndocrinology
Volume157
Issue number3
DOIs
StatePublished - Mar 1 2016

Fingerprint

Mesenchymal Stromal Cells
Adipocytes
Osteoblasts
Adipogenesis
Zinc Fingers
Hematopoiesis
Cell Lineage
Transcription Factors
Cell Proliferation

ASJC Scopus subject areas

  • Endocrinology

Cite this

Gata2 is a rheostat for mesenchymal stem cell fate in male mice. / Li, Xiaoxiao; Huynh, Hoangdinh; Zuo, Hao; Salminen, Marjo; Wan, Yihong.

In: Endocrinology, Vol. 157, No. 3, 01.03.2016, p. 1021-1028.

Research output: Contribution to journalArticle

Li, X, Huynh, H, Zuo, H, Salminen, M & Wan, Y 2016, 'Gata2 is a rheostat for mesenchymal stem cell fate in male mice', Endocrinology, vol. 157, no. 3, pp. 1021-1028. https://doi.org/10.1210/en.2015-1827
Li, Xiaoxiao ; Huynh, Hoangdinh ; Zuo, Hao ; Salminen, Marjo ; Wan, Yihong. / Gata2 is a rheostat for mesenchymal stem cell fate in male mice. In: Endocrinology. 2016 ; Vol. 157, No. 3. pp. 1021-1028.
@article{ee34d945f5114a498edc60f9dc04ef9e,
title = "Gata2 is a rheostat for mesenchymal stem cell fate in male mice",
abstract = "Gata2 is a zinc finger transcription factor that is important in hematopoiesis and neuronal development. However, the roles of Gata2 in the mesenchymal lineages are poorly understood. In vitro studies suggest that Gata2 modulates adipocyte differentiation and mesenchymal stem cell (MSC) proliferation. To systematically determine the in vivo functions of Gata2 in the MSC lineage commitment and development, we have generated three mouse models in which Gata2 is specifically deleted in MSCs, adipocytes, or osteoblasts. During the MSC expansion stage, Gata2 promotes proliferation and attenuates differentiation; thereby Gata2 loss in MSCs results in enhanced differentiation of both adipocytes and osteoblasts. During the differentiation stage, Gata2 also plays MSC-independent roles to impede lineage commitment; hence, Gata2 loss in adipocyte or osteoblast lineages also augments adipogenesis and osteoblastogenesis, respectively. These findings reveal Gata2 as a crucial rheostat of MSC fate to control osteoblast and adipocyte lineage development.",
author = "Xiaoxiao Li and Hoangdinh Huynh and Hao Zuo and Marjo Salminen and Yihong Wan",
year = "2016",
month = "3",
day = "1",
doi = "10.1210/en.2015-1827",
language = "English (US)",
volume = "157",
pages = "1021--1028",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "3",

}

TY - JOUR

T1 - Gata2 is a rheostat for mesenchymal stem cell fate in male mice

AU - Li, Xiaoxiao

AU - Huynh, Hoangdinh

AU - Zuo, Hao

AU - Salminen, Marjo

AU - Wan, Yihong

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Gata2 is a zinc finger transcription factor that is important in hematopoiesis and neuronal development. However, the roles of Gata2 in the mesenchymal lineages are poorly understood. In vitro studies suggest that Gata2 modulates adipocyte differentiation and mesenchymal stem cell (MSC) proliferation. To systematically determine the in vivo functions of Gata2 in the MSC lineage commitment and development, we have generated three mouse models in which Gata2 is specifically deleted in MSCs, adipocytes, or osteoblasts. During the MSC expansion stage, Gata2 promotes proliferation and attenuates differentiation; thereby Gata2 loss in MSCs results in enhanced differentiation of both adipocytes and osteoblasts. During the differentiation stage, Gata2 also plays MSC-independent roles to impede lineage commitment; hence, Gata2 loss in adipocyte or osteoblast lineages also augments adipogenesis and osteoblastogenesis, respectively. These findings reveal Gata2 as a crucial rheostat of MSC fate to control osteoblast and adipocyte lineage development.

AB - Gata2 is a zinc finger transcription factor that is important in hematopoiesis and neuronal development. However, the roles of Gata2 in the mesenchymal lineages are poorly understood. In vitro studies suggest that Gata2 modulates adipocyte differentiation and mesenchymal stem cell (MSC) proliferation. To systematically determine the in vivo functions of Gata2 in the MSC lineage commitment and development, we have generated three mouse models in which Gata2 is specifically deleted in MSCs, adipocytes, or osteoblasts. During the MSC expansion stage, Gata2 promotes proliferation and attenuates differentiation; thereby Gata2 loss in MSCs results in enhanced differentiation of both adipocytes and osteoblasts. During the differentiation stage, Gata2 also plays MSC-independent roles to impede lineage commitment; hence, Gata2 loss in adipocyte or osteoblast lineages also augments adipogenesis and osteoblastogenesis, respectively. These findings reveal Gata2 as a crucial rheostat of MSC fate to control osteoblast and adipocyte lineage development.

UR - http://www.scopus.com/inward/record.url?scp=84960485263&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84960485263&partnerID=8YFLogxK

U2 - 10.1210/en.2015-1827

DO - 10.1210/en.2015-1827

M3 - Article

C2 - 26812161

AN - SCOPUS:84960485263

VL - 157

SP - 1021

EP - 1028

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 3

ER -