Gelsolin inhibition of fast axonal transport indicates a requirement for actin microfilaments

Scott T. Brady, Raymond J. Lasek, Robert D. Allen, Helen L. Yin, Thomas P. Stossel

Research output: Contribution to journalArticle

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Abstract

The actions of actin-based microfilaments in cell motility suggest a possible role in the mechanism of fast axonal transport1-3, but the pharmacological data evaluating their role in this process are equivocal 4-6. Moreover, microfilaments are difficult to preserve and identify in ultrastructural studies7, so the organization and function of axonal actin has remained uncertain. We have now evaluated the role of actin microfilaments in intracellular transport of membranous organelles using video-enhanced contrast microscopy and gelsolin to analyse fast axonal transport directly in isolated axoplasm from the squid giant axon. With this preparation it is possible to perfuse axoplasm with large molecules that do not cross the plasmalemma, while controlling cation levels. The 90,000-molecular weight protein gelsolin depolymerizes actin microfilaments in micromolar Ca 2+, but not in the absence of Ca2+. Axonal transport of membranous organelles has previously been shown to be unaffected by levels of Ca2+ up to 10 M8. In the presence of EGTA, gelsolin has no effect on the movement of membranous organelles, but in the presence of 10 M Ca2+ it completely blocks transport of all membranous organelles. No changes in the organization of the axoplasm were detected. These results and results using other probes for actin are consistent with the hypothesis that actin-based microfilaments are involved in the movement of membranous organelles in the axon.

Original languageEnglish (US)
Pages (from-to)56-58
Number of pages3
JournalNature
Volume310
Issue number5972
StatePublished - 1984

Fingerprint

Gelsolin
Axonal Transport
Actin Cytoskeleton
Organelles
Axons
Actins
Decapodiformes
Egtazic Acid
Cell Movement
Cations
Microscopy
Molecular Weight
Pharmacology

ASJC Scopus subject areas

  • General

Cite this

Brady, S. T., Lasek, R. J., Allen, R. D., Yin, H. L., & Stossel, T. P. (1984). Gelsolin inhibition of fast axonal transport indicates a requirement for actin microfilaments. Nature, 310(5972), 56-58.

Gelsolin inhibition of fast axonal transport indicates a requirement for actin microfilaments. / Brady, Scott T.; Lasek, Raymond J.; Allen, Robert D.; Yin, Helen L.; Stossel, Thomas P.

In: Nature, Vol. 310, No. 5972, 1984, p. 56-58.

Research output: Contribution to journalArticle

Brady, ST, Lasek, RJ, Allen, RD, Yin, HL & Stossel, TP 1984, 'Gelsolin inhibition of fast axonal transport indicates a requirement for actin microfilaments', Nature, vol. 310, no. 5972, pp. 56-58.
Brady, Scott T. ; Lasek, Raymond J. ; Allen, Robert D. ; Yin, Helen L. ; Stossel, Thomas P. / Gelsolin inhibition of fast axonal transport indicates a requirement for actin microfilaments. In: Nature. 1984 ; Vol. 310, No. 5972. pp. 56-58.
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