Gene complementing xeroderma pigmentosum group A cells maps to distal human chromosome 9q

Karla A. Henning; Roger A. Schultz; Gurbax S. Sekhon; Errol C. Friedberg

doi:10.1007/BF01232467

Gene complementing xeroderma pigmentosum group A cells maps to distal human chromosome 9q

Karla A. Henning, Roger A. Schultz, Gurbax S. Sekhon, Errol C. Friedberg

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Abstract

Phenotypic complementation of xeroderma pigmentosum group A (XP-A) cells by microcell-mediated transfer of a single rearranged neo-tagged human chromosome from a human-mouse somatic cell hybrid designated K3SUB1A9-3 was reported previously. Extended growth of this human-mouse hybrid in culture led to deletion of the small arm of the human chromosome, with concomitant loss of complementing ability when introduced into XP-A cells by microcell-mediated chromosome transfer. Cytogenetic analysis of both hybrids suggests that the complementing locus is on chromosome 9q22.2-q34.3, and Southern blot analysis confirms the presence of distal chromosome 9q sequences.

Original language	English (US)
Pages (from-to)	395-400
Number of pages	6
Journal	Somatic Cell and Molecular Genetics
Volume	16
Issue number	4
DOIs	https://doi.org/10.1007/BF01232467
State	Published - Jul 1990

ASJC Scopus subject areas

Genetics
Cell Biology

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title = "Gene complementing xeroderma pigmentosum group A cells maps to distal human chromosome 9q",

abstract = "Phenotypic complementation of xeroderma pigmentosum group A (XP-A) cells by microcell-mediated transfer of a single rearranged neo-tagged human chromosome from a human-mouse somatic cell hybrid designated K3SUB1A9-3 was reported previously. Extended growth of this human-mouse hybrid in culture led to deletion of the small arm of the human chromosome, with concomitant loss of complementing ability when introduced into XP-A cells by microcell-mediated chromosome transfer. Cytogenetic analysis of both hybrids suggests that the complementing locus is on chromosome 9q22.2-q34.3, and Southern blot analysis confirms the presence of distal chromosome 9q sequences.",

author = "Henning, {Karla A.} and Schultz, {Roger A.} and Sekhon, {Gurbax S.} and Friedberg, {Errol C.}",

year = "1990",

month = jul,

doi = "10.1007/BF01232467",

language = "English (US)",

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journal = "Somatic Cell and Molecular Genetics",

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T1 - Gene complementing xeroderma pigmentosum group A cells maps to distal human chromosome 9q

AU - Henning, Karla A.

AU - Schultz, Roger A.

AU - Sekhon, Gurbax S.

AU - Friedberg, Errol C.

PY - 1990/7

Y1 - 1990/7

N2 - Phenotypic complementation of xeroderma pigmentosum group A (XP-A) cells by microcell-mediated transfer of a single rearranged neo-tagged human chromosome from a human-mouse somatic cell hybrid designated K3SUB1A9-3 was reported previously. Extended growth of this human-mouse hybrid in culture led to deletion of the small arm of the human chromosome, with concomitant loss of complementing ability when introduced into XP-A cells by microcell-mediated chromosome transfer. Cytogenetic analysis of both hybrids suggests that the complementing locus is on chromosome 9q22.2-q34.3, and Southern blot analysis confirms the presence of distal chromosome 9q sequences.

AB - Phenotypic complementation of xeroderma pigmentosum group A (XP-A) cells by microcell-mediated transfer of a single rearranged neo-tagged human chromosome from a human-mouse somatic cell hybrid designated K3SUB1A9-3 was reported previously. Extended growth of this human-mouse hybrid in culture led to deletion of the small arm of the human chromosome, with concomitant loss of complementing ability when introduced into XP-A cells by microcell-mediated chromosome transfer. Cytogenetic analysis of both hybrids suggests that the complementing locus is on chromosome 9q22.2-q34.3, and Southern blot analysis confirms the presence of distal chromosome 9q sequences.

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Gene complementing xeroderma pigmentosum group A cells maps to distal human chromosome 9q

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