Gene Expression Analysis Identifies Cholesterol Metabolism Dysregulation in Hippocampus of Phenytoin-Resistant Pentylenetetrazol-Kindled Epileptic Mice

Sridhar Amalakanti; Unis Ahmad Bhat; Madhavi B. Mylavarapu; Nitin Khandelwal; N. V. Sundarachary; Sumana Chakravarty; Arvind Kumar

doi:10.1007/s12017-021-08648-0

Gene Expression Analysis Identifies Cholesterol Metabolism Dysregulation in Hippocampus of Phenytoin-Resistant Pentylenetetrazol-Kindled Epileptic Mice

Sridhar Amalakanti, Unis Ahmad Bhat, Madhavi B. Mylavarapu, Nitin Khandelwal, N. V. Sundarachary, Sumana Chakravarty, Arvind Kumar

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Pharmaco-resistant Epilepsy has been a major challenge for medical interventions in controlling seizures. To date, up to 33% of the patients with epilepsy do not show adequate response to anti-epileptic drugs even after prolonged combinatorial drug usage. Using microarray, this study explores the changes in hippocampal gene expression in the phenytoin-resistant pentylenetetrazol (PTZ)-kindled mouse model of epilepsy. Our results from mRNA microarray analysis show distinct gene expression profiles in the hippocampus of phenytoin-resistant and sensitive mice. Pathway enrichment analysis showed differential expression of genes involved in cholesterol biosynthesis in phenytoin-resistant and sensitive mice.

Original language	English (US)
Pages (from-to)	485-490
Number of pages	6
Journal	NeuroMolecular Medicine
Volume	23
Issue number	4
DOIs	https://doi.org/10.1007/s12017-021-08648-0
State	Published - Dec 2021
Externally published	Yes

Keywords

Cholesterol
Convulsion
Drug-resistant epilepsy
Microarray
Neurological

ASJC Scopus subject areas

Molecular Medicine
Neurology
Cellular and Molecular Neuroscience

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10.1007/s12017-021-08648-0

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title = "Gene Expression Analysis Identifies Cholesterol Metabolism Dysregulation in Hippocampus of Phenytoin-Resistant Pentylenetetrazol-Kindled Epileptic Mice",

abstract = "Pharmaco-resistant Epilepsy has been a major challenge for medical interventions in controlling seizures. To date, up to 33% of the patients with epilepsy do not show adequate response to anti-epileptic drugs even after prolonged combinatorial drug usage. Using microarray, this study explores the changes in hippocampal gene expression in the phenytoin-resistant pentylenetetrazol (PTZ)-kindled mouse model of epilepsy. Our results from mRNA microarray analysis show distinct gene expression profiles in the hippocampus of phenytoin-resistant and sensitive mice. Pathway enrichment analysis showed differential expression of genes involved in cholesterol biosynthesis in phenytoin-resistant and sensitive mice.",

keywords = "Cholesterol, Convulsion, Drug-resistant epilepsy, Microarray, Neurological",

author = "Sridhar Amalakanti and Bhat, {Unis Ahmad} and Mylavarapu, {Madhavi B.} and Nitin Khandelwal and Sundarachary, {N. V.} and Sumana Chakravarty and Arvind Kumar",

note = "Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.",

year = "2021",

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doi = "10.1007/s12017-021-08648-0",

language = "English (US)",

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AU - Bhat, Unis Ahmad

AU - Mylavarapu, Madhavi B.

AU - Khandelwal, Nitin

AU - Sundarachary, N. V.

AU - Chakravarty, Sumana

AU - Kumar, Arvind

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AB - Pharmaco-resistant Epilepsy has been a major challenge for medical interventions in controlling seizures. To date, up to 33% of the patients with epilepsy do not show adequate response to anti-epileptic drugs even after prolonged combinatorial drug usage. Using microarray, this study explores the changes in hippocampal gene expression in the phenytoin-resistant pentylenetetrazol (PTZ)-kindled mouse model of epilepsy. Our results from mRNA microarray analysis show distinct gene expression profiles in the hippocampus of phenytoin-resistant and sensitive mice. Pathway enrichment analysis showed differential expression of genes involved in cholesterol biosynthesis in phenytoin-resistant and sensitive mice.

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Gene Expression Analysis Identifies Cholesterol Metabolism Dysregulation in Hippocampus of Phenytoin-Resistant Pentylenetetrazol-Kindled Epileptic Mice

Abstract

Keywords

ASJC Scopus subject areas

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