Gene expression analysis of dupuytren's disease: The role of TGF-β2

Andrew Y. Zhang, K. D. Fong, H. Pham, R. P. Nacamuli, M. T. Longaker, J. Chang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Dupuytren's disease is characterised by nodular fibroblastic proliferation of the palmar fascia leading to contracture of the hand. Transforming growth factor beta (TGF-β) is thought to play a role in its pathogenesis. We performed a cDNA microarray analysis of Dupuytren's diseased cord tissue with an emphasis on TGF-β isoforms. Normal-appearing transverse ligament of the palmar fascia from adjacent to the diseased cord and palmar fascia from patients undergoing carpal tunnel release were used as controls. TGF-β gene expression was confirmed by quantitative real-time polymerase chain reaction. Over 20 unique genes were found to be significantly up-regulated, including several previously reported genes. A dominant increase in TGF-β2 expression was seen in the cord tissue, whereas TGF-β1 and TGF-β3 were found not to be significantly up-regulated. Quantitative real-time polymerase chain reaction confirmed these findings. This gene expression profile allows for further experiments that may eventually lead to gene therapy to block the development and progression of Dupuytren's disease clinically.

Original languageEnglish (US)
Pages (from-to)783-790
Number of pages8
JournalJournal of Hand Surgery: European Volume
Volume33
Issue number6
DOIs
StatePublished - Dec 1 2008

Fingerprint

Dupuytren Contracture
Transforming Growth Factor beta
Gene Expression
Fascia
Real-Time Polymerase Chain Reaction
Contracture
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Wrist
Transcriptome
Ligaments
Genetic Therapy
Genes
Protein Isoforms
Hand

Keywords

  • Dupuytren's disease
  • Microarray analysis
  • TGF-β gene expression

ASJC Scopus subject areas

  • Surgery

Cite this

Gene expression analysis of dupuytren's disease : The role of TGF-β2. / Zhang, Andrew Y.; Fong, K. D.; Pham, H.; Nacamuli, R. P.; Longaker, M. T.; Chang, J.

In: Journal of Hand Surgery: European Volume, Vol. 33, No. 6, 01.12.2008, p. 783-790.

Research output: Contribution to journalArticle

Zhang, Andrew Y. ; Fong, K. D. ; Pham, H. ; Nacamuli, R. P. ; Longaker, M. T. ; Chang, J. / Gene expression analysis of dupuytren's disease : The role of TGF-β2. In: Journal of Hand Surgery: European Volume. 2008 ; Vol. 33, No. 6. pp. 783-790.
@article{e683640632c4475da14da7d47d8ea1f2,
title = "Gene expression analysis of dupuytren's disease: The role of TGF-β2",
abstract = "Dupuytren's disease is characterised by nodular fibroblastic proliferation of the palmar fascia leading to contracture of the hand. Transforming growth factor beta (TGF-β) is thought to play a role in its pathogenesis. We performed a cDNA microarray analysis of Dupuytren's diseased cord tissue with an emphasis on TGF-β isoforms. Normal-appearing transverse ligament of the palmar fascia from adjacent to the diseased cord and palmar fascia from patients undergoing carpal tunnel release were used as controls. TGF-β gene expression was confirmed by quantitative real-time polymerase chain reaction. Over 20 unique genes were found to be significantly up-regulated, including several previously reported genes. A dominant increase in TGF-β2 expression was seen in the cord tissue, whereas TGF-β1 and TGF-β3 were found not to be significantly up-regulated. Quantitative real-time polymerase chain reaction confirmed these findings. This gene expression profile allows for further experiments that may eventually lead to gene therapy to block the development and progression of Dupuytren's disease clinically.",
keywords = "Dupuytren's disease, Microarray analysis, TGF-β gene expression",
author = "Zhang, {Andrew Y.} and Fong, {K. D.} and H. Pham and Nacamuli, {R. P.} and Longaker, {M. T.} and J. Chang",
year = "2008",
month = "12",
day = "1",
doi = "10.1177/1753193408091352",
language = "English (US)",
volume = "33",
pages = "783--790",
journal = "The Hand",
issn = "1753-1934",
publisher = "SAGE Publications Ltd",
number = "6",

}

TY - JOUR

T1 - Gene expression analysis of dupuytren's disease

T2 - The role of TGF-β2

AU - Zhang, Andrew Y.

AU - Fong, K. D.

AU - Pham, H.

AU - Nacamuli, R. P.

AU - Longaker, M. T.

AU - Chang, J.

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Dupuytren's disease is characterised by nodular fibroblastic proliferation of the palmar fascia leading to contracture of the hand. Transforming growth factor beta (TGF-β) is thought to play a role in its pathogenesis. We performed a cDNA microarray analysis of Dupuytren's diseased cord tissue with an emphasis on TGF-β isoforms. Normal-appearing transverse ligament of the palmar fascia from adjacent to the diseased cord and palmar fascia from patients undergoing carpal tunnel release were used as controls. TGF-β gene expression was confirmed by quantitative real-time polymerase chain reaction. Over 20 unique genes were found to be significantly up-regulated, including several previously reported genes. A dominant increase in TGF-β2 expression was seen in the cord tissue, whereas TGF-β1 and TGF-β3 were found not to be significantly up-regulated. Quantitative real-time polymerase chain reaction confirmed these findings. This gene expression profile allows for further experiments that may eventually lead to gene therapy to block the development and progression of Dupuytren's disease clinically.

AB - Dupuytren's disease is characterised by nodular fibroblastic proliferation of the palmar fascia leading to contracture of the hand. Transforming growth factor beta (TGF-β) is thought to play a role in its pathogenesis. We performed a cDNA microarray analysis of Dupuytren's diseased cord tissue with an emphasis on TGF-β isoforms. Normal-appearing transverse ligament of the palmar fascia from adjacent to the diseased cord and palmar fascia from patients undergoing carpal tunnel release were used as controls. TGF-β gene expression was confirmed by quantitative real-time polymerase chain reaction. Over 20 unique genes were found to be significantly up-regulated, including several previously reported genes. A dominant increase in TGF-β2 expression was seen in the cord tissue, whereas TGF-β1 and TGF-β3 were found not to be significantly up-regulated. Quantitative real-time polymerase chain reaction confirmed these findings. This gene expression profile allows for further experiments that may eventually lead to gene therapy to block the development and progression of Dupuytren's disease clinically.

KW - Dupuytren's disease

KW - Microarray analysis

KW - TGF-β gene expression

UR - http://www.scopus.com/inward/record.url?scp=59749097505&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=59749097505&partnerID=8YFLogxK

U2 - 10.1177/1753193408091352

DO - 10.1177/1753193408091352

M3 - Article

C2 - 18694919

AN - SCOPUS:59749097505

VL - 33

SP - 783

EP - 790

JO - The Hand

JF - The Hand

SN - 1753-1934

IS - 6

ER -