Gene expression changes in normal human skin fibroblasts induced by HZE-particle radiation

Liang Hao Ding, Masato Shingyoji, Fanqing Chen, Aloke Chatterjee, Kiyomi Eguchi Kasai, David J. Chen

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Studies have shown that radiation exposure affects global gene expression in mammalian cells. However, little is known about the effects of HZE particles on gene expression. To study these effects, human skin fibroblasts were irradiated with HZE particles of different energies and LETs. The data obtained from these experiments indicate that changes in gene expression are dependent on the energy of the radiation source. Particles with the highest energy, i.e. iron, induced the biggest expression changes in terms of numbers of genes and magnitudes of changes. Many genes were found to undergo significant expression changes after HZE-particle irradiation, including CDKN1A/p21, MDM2, TNFRSF6/fas, PCNA and RAD52. Unlike X rays, HZE particles expose cells to two types of radiation: primary ions and δ rays. We hypothesized that the biological effects of δ rays, which are secondary electron emissions, should resemble the effects of X rays. To explore this idea, gene expression changes between cells that had been irradiated with HZE particles and X rays were compared. The results support our hypothesis since the number of genes that commonly changed after exposure to both radiations increased as a function of particle energy.

Original languageEnglish (US)
Pages (from-to)523-526
Number of pages4
JournalRadiation Research
Volume164
Issue number4 II
DOIs
StatePublished - Oct 2005

Fingerprint

Cosmic Radiation
gene expression
fibroblasts
skin (animal)
Fibroblasts
Radiation
Gene Expression
Skin
radiation
genes
X-Rays
rays
X-radiation
energy
Genes
Linear Energy Transfer
Proliferating Cell Nuclear Antigen
x rays
biological effects
radiation dosage

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Ding, L. H., Shingyoji, M., Chen, F., Chatterjee, A., Kasai, K. E., & Chen, D. J. (2005). Gene expression changes in normal human skin fibroblasts induced by HZE-particle radiation. Radiation Research, 164(4 II), 523-526. https://doi.org/10.1667/RR3350.1

Gene expression changes in normal human skin fibroblasts induced by HZE-particle radiation. / Ding, Liang Hao; Shingyoji, Masato; Chen, Fanqing; Chatterjee, Aloke; Kasai, Kiyomi Eguchi; Chen, David J.

In: Radiation Research, Vol. 164, No. 4 II, 10.2005, p. 523-526.

Research output: Contribution to journalArticle

Ding, LH, Shingyoji, M, Chen, F, Chatterjee, A, Kasai, KE & Chen, DJ 2005, 'Gene expression changes in normal human skin fibroblasts induced by HZE-particle radiation', Radiation Research, vol. 164, no. 4 II, pp. 523-526. https://doi.org/10.1667/RR3350.1
Ding, Liang Hao ; Shingyoji, Masato ; Chen, Fanqing ; Chatterjee, Aloke ; Kasai, Kiyomi Eguchi ; Chen, David J. / Gene expression changes in normal human skin fibroblasts induced by HZE-particle radiation. In: Radiation Research. 2005 ; Vol. 164, No. 4 II. pp. 523-526.
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