Gene expression of Endothelial type isoform of nitric oxide synthase in various tissues of stroke-prone spontaneously hypertensive rats

T. Seki, M. Naruse, K. Naruse, T. Katafuchi, K. M. Lodhi, T. Yoshimoto, H. Hagiwara, H. Demura, S. Hirose

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Nitric oxide (NO) has been suggested to play important roles in the pathophysiology of various cardiovascular diseases. This study tested the hypothesis that an attenuated biological action of NO in hypertension is attributed to a change in the gene expression of NO synthase (NOS), a key enzyme involved in NO formation. The expression level of mRNA of endothelial type NOS (NOS-III) was determined in stroke-prone spontaneously hypertensive rats (SHR-SP/Izm) and Wistar Kyoto rats (WKY/Izm) by ribonuclease protection assay using a partial clone as probe, NOS-III mRNA was expressed ubiquitously in various tissues of WKY/Izm and SHR-SP/Izm either at 5 wk or 13 wk of age. There was no significant difference in the tissue expression of NOS-III mRNA between the two strains at either age. The intensity and localization of the hybridization signal for NOS-III mRNA in the heart of SHR-SP/Izm did not differ from those in the heart of WKY/Izm. These results suggest that the attenuated biological action of NO implied in genetically hypertensive rats is not attributed to an abnormality at the level of NOS-III mRNA expression in the tissues, although lack of an increase in NOS-III gene expression, despite the hypertensive hemodynamic stress, may modify the blood pressure in hypertension.

Original languageEnglish (US)
Pages (from-to)43-49
Number of pages7
JournalHypertension Research - Clinical and Experimental
Volume20
Issue number1
StatePublished - 1997

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Nitric Oxide Synthase Type III
Inbred SHR Rats
Nitric Oxide Synthase
Protein Isoforms
Stroke
Gene Expression
Nitric Oxide
Messenger RNA
Hypertension
Inbred WKY Rats
Ribonucleases
Cardiovascular Diseases
Clone Cells
Hemodynamics
Blood Pressure
Enzymes

Keywords

  • Gene expression
  • Nitric oxide
  • Nitric oxide synthase
  • Stroke-prone spontaneously hypertensive rats

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Gene expression of Endothelial type isoform of nitric oxide synthase in various tissues of stroke-prone spontaneously hypertensive rats. / Seki, T.; Naruse, M.; Naruse, K.; Katafuchi, T.; Lodhi, K. M.; Yoshimoto, T.; Hagiwara, H.; Demura, H.; Hirose, S.

In: Hypertension Research - Clinical and Experimental, Vol. 20, No. 1, 1997, p. 43-49.

Research output: Contribution to journalArticle

Seki, T, Naruse, M, Naruse, K, Katafuchi, T, Lodhi, KM, Yoshimoto, T, Hagiwara, H, Demura, H & Hirose, S 1997, 'Gene expression of Endothelial type isoform of nitric oxide synthase in various tissues of stroke-prone spontaneously hypertensive rats', Hypertension Research - Clinical and Experimental, vol. 20, no. 1, pp. 43-49.
Seki, T. ; Naruse, M. ; Naruse, K. ; Katafuchi, T. ; Lodhi, K. M. ; Yoshimoto, T. ; Hagiwara, H. ; Demura, H. ; Hirose, S. / Gene expression of Endothelial type isoform of nitric oxide synthase in various tissues of stroke-prone spontaneously hypertensive rats. In: Hypertension Research - Clinical and Experimental. 1997 ; Vol. 20, No. 1. pp. 43-49.
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AU - Yoshimoto, T.

AU - Hagiwara, H.

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AU - Hirose, S.

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