TY - JOUR
T1 - Gene isolation with cDNA probes from identified aplysia neurons
T2 - Neuropeptide modulators of cardiovascular physiology
AU - Nambu, John R.
AU - Taussig, Ronald
AU - Mahon, Anne C.
AU - Scheller, Richard H.
N1 - Funding Information:
We would like to thank Drs . Barry Rothman, Randy Johnson, Karen Sigvart, Robert Ho, and Earl Mayeri for help with the dissections . The authors wish to thank Corey Goodman and Paul Taghert for the critical reading of this manuscript . We are indebted to Michael Palazolo, James Schwartz, and Richard Axel for use of an abdominal gangion cDNA library . The secretarial assistance of Linda Harwood was invaluable . Anne Mahon is a National Institutes of Health postdoctoral fellow . This work was supported by start-up funds from the Department of Biological Sciences, as well as by institutional grants to Stanford University from the American Cancer Society and NIH . Additional support from the McKnight Foundation and NIH (R01 NS 19542) is greatly appreciated .
PY - 1983/11
Y1 - 1983/11
N2 - The Aplysia abdominal ganglion neurons, R3-R14, modulate cardiovascular activity. In vitro translations of poly(A)+ RNA from these cells suggest that they contain a prevalent mRNA encoding a 14 kd protein. Utilizing differential screening techniques with 32P-labeled cDNA synthesized from the poly(A)+ RNA of identified neurons, we isolated the corresponding gene. The Aplysia haploid genome contains a single copy of this sequence, which is interrupted by two large introns and spans ∼7 kb of genomic DNA. The R3-R14 neurons specifically express this gene, resulting in the synthesis of a 1.25 kb mRNA not found in other abdominal ganglion cells or in the head ganglia. The gene was shown to encode a 13.5 kd precursor, which is proteolytically cleaved into at least three peptides with molecular weights of 5.0, 3.3, and 1.3 kd. These peptides and glycine are thought to act as chemical messengers in the central nervous system and peripherally.
AB - The Aplysia abdominal ganglion neurons, R3-R14, modulate cardiovascular activity. In vitro translations of poly(A)+ RNA from these cells suggest that they contain a prevalent mRNA encoding a 14 kd protein. Utilizing differential screening techniques with 32P-labeled cDNA synthesized from the poly(A)+ RNA of identified neurons, we isolated the corresponding gene. The Aplysia haploid genome contains a single copy of this sequence, which is interrupted by two large introns and spans ∼7 kb of genomic DNA. The R3-R14 neurons specifically express this gene, resulting in the synthesis of a 1.25 kb mRNA not found in other abdominal ganglion cells or in the head ganglia. The gene was shown to encode a 13.5 kd precursor, which is proteolytically cleaved into at least three peptides with molecular weights of 5.0, 3.3, and 1.3 kd. These peptides and glycine are thought to act as chemical messengers in the central nervous system and peripherally.
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U2 - 10.1016/0092-8674(83)90206-4
DO - 10.1016/0092-8674(83)90206-4
M3 - Article
C2 - 6194901
AN - SCOPUS:0021030232
SN - 0092-8674
VL - 35
SP - 47
EP - 56
JO - Cell
JF - Cell
IS - 1
ER -