Gene isolation with cDNA probes from identified aplysia neurons: Neuropeptide modulators of cardiovascular physiology

John R. Nambu, Ronald Taussig, Anne C. Mahon, Richard H. Scheller

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

The Aplysia abdominal ganglion neurons, R3-R14, modulate cardiovascular activity. In vitro translations of poly(A)+ RNA from these cells suggest that they contain a prevalent mRNA encoding a 14 kd protein. Utilizing differential screening techniques with 32P-labeled cDNA synthesized from the poly(A)+ RNA of identified neurons, we isolated the corresponding gene. The Aplysia haploid genome contains a single copy of this sequence, which is interrupted by two large introns and spans ∼7 kb of genomic DNA. The R3-R14 neurons specifically express this gene, resulting in the synthesis of a 1.25 kb mRNA not found in other abdominal ganglion cells or in the head ganglia. The gene was shown to encode a 13.5 kd precursor, which is proteolytically cleaved into at least three peptides with molecular weights of 5.0, 3.3, and 1.3 kd. These peptides and glycine are thought to act as chemical messengers in the central nervous system and peripherally.

Original languageEnglish (US)
Pages (from-to)47-56
Number of pages10
JournalCell
Volume35
Issue number1
DOIs
StatePublished - Nov 1983

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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