Gene-targeted metagenomic analysis of glucan-branching enzyme gene profiles among human and animal fecal microbiota

Sunghee Lee, Brandi Cantarel, Bernard Henrissat, Dirk Gevers, Bruce W. Birren, Curtis Huttenhower, Gwangpyo Ko

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Glycoside hydrolases (GHs), the enzymes that breakdown complex carbohydrates, are a highly diversified class of key enzymes associated with the gut microbiota and its metabolic functions. To learn more about the diversity of GHs and their potential role in a variety of gut microbiomes, we used a combination of 16S, metagenomic and targeted amplicon sequencing data to study one of these enzyme families in detail. Specifically, we employed a functional gene-targeted metagenomic approach to the 1-4-α-glucan-branching enzyme (gBE) gene in the gut microbiomes of four host species (human, chicken, cow and pig). The characteristics of operational taxonomic units (OTUs) and operational glucan-branching units (OGBUs) were distinctive in each of hosts. Human and pig were most similar in OTUs profiles while maintaining distinct OGBU profiles. Interestingly, the phylogenetic profiles identified from 16S and gBE gene sequences differed, suggesting the presence of different gBE genes in the same OTU across different vertebrate hosts. Our data suggest that gene-targeted metagenomic analysis is useful for an in-depth understanding of the diversity of a particular gene of interest. Specific carbohydrate metabolic genes appear to be carried by distinct OTUs in different individual hosts and among different vertebrate species' microbiomes, the characteristics of which differ according to host genetic background and/or diet.

Original languageEnglish (US)
Pages (from-to)493-503
Number of pages11
JournalISME Journal
Volume8
Issue number3
DOIs
StatePublished - Mar 1 2014

Fingerprint

1,4-alpha-Glucan Branching Enzyme
Metagenomics
Glucans
Microbiota
glucans
branching
enzyme
gene
animal
enzymes
Genes
animals
genes
hydrolases
Glycoside Hydrolases
glycosides
pig
digestive system
Vertebrates
vertebrates

Keywords

  • 1-4-α-glucan-branching enzyme (gBE) gene
  • carbohydrate metabolism
  • gene-targeted metagenomics
  • glycoside hydrolase family 13
  • gut microbiota

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Gene-targeted metagenomic analysis of glucan-branching enzyme gene profiles among human and animal fecal microbiota. / Lee, Sunghee; Cantarel, Brandi; Henrissat, Bernard; Gevers, Dirk; Birren, Bruce W.; Huttenhower, Curtis; Ko, Gwangpyo.

In: ISME Journal, Vol. 8, No. 3, 01.03.2014, p. 493-503.

Research output: Contribution to journalArticle

Lee, Sunghee ; Cantarel, Brandi ; Henrissat, Bernard ; Gevers, Dirk ; Birren, Bruce W. ; Huttenhower, Curtis ; Ko, Gwangpyo. / Gene-targeted metagenomic analysis of glucan-branching enzyme gene profiles among human and animal fecal microbiota. In: ISME Journal. 2014 ; Vol. 8, No. 3. pp. 493-503.
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