Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector

Drake Laface, Paul Hermonat, Edward Wakeland, Ammon Peck

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formation in the presence of geneticin sulfate (G-418). The value of AAV as an alternative to the retrovirus vector systems is discussed.

Original languageEnglish (US)
Pages (from-to)483-486
Number of pages4
JournalVirology
Volume162
Issue number2
DOIs
StatePublished - 1988

Fingerprint

Dependovirus
Hematopoietic Stem Cells
Drug Resistance
Neomycin
Retroviridae
Genes
Stem Cells
antibiotic G 418

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector. / Laface, Drake; Hermonat, Paul; Wakeland, Edward; Peck, Ammon.

In: Virology, Vol. 162, No. 2, 1988, p. 483-486.

Research output: Contribution to journalArticle

Laface, Drake ; Hermonat, Paul ; Wakeland, Edward ; Peck, Ammon. / Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector. In: Virology. 1988 ; Vol. 162, No. 2. pp. 483-486.
@article{585767018b724cc79fcdc806801d9ef2,
title = "Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector",
abstract = "We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5{\%} by colony formation in the presence of geneticin sulfate (G-418). The value of AAV as an alternative to the retrovirus vector systems is discussed.",
author = "Drake Laface and Paul Hermonat and Edward Wakeland and Ammon Peck",
year = "1988",
doi = "10.1016/0042-6822(88)90491-6",
language = "English (US)",
volume = "162",
pages = "483--486",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector

AU - Laface, Drake

AU - Hermonat, Paul

AU - Wakeland, Edward

AU - Peck, Ammon

PY - 1988

Y1 - 1988

N2 - We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formation in the presence of geneticin sulfate (G-418). The value of AAV as an alternative to the retrovirus vector systems is discussed.

AB - We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formation in the presence of geneticin sulfate (G-418). The value of AAV as an alternative to the retrovirus vector systems is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0023953618&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023953618&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(88)90491-6

DO - 10.1016/0042-6822(88)90491-6

M3 - Article

C2 - 2829430

AN - SCOPUS:0023953618

VL - 162

SP - 483

EP - 486

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -