Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector

Drake Laface; Paul Hermonat; Edward Wakeland; Ammon Peck

doi:10.1016/0042-6822(88)90491-6

Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector

Drake Laface, Paul Hermonat, Edward Wakeland, Ammon Peck

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formation in the presence of geneticin sulfate (G-418). The value of AAV as an alternative to the retrovirus vector systems is discussed.

Original language	English (US)
Pages (from-to)	483-486
Number of pages	4
Journal	Virology
Volume	162
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(88)90491-6
State	Published - Feb 1988

ASJC Scopus subject areas

Virology

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10.1016/0042-6822(88)90491-6

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title = "Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector",

abstract = "We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formation in the presence of geneticin sulfate (G-418). The value of AAV as an alternative to the retrovirus vector systems is discussed.",

author = "Drake Laface and Paul Hermonat and Edward Wakeland and Ammon Peck",

note = "Funding Information: We thank Dr. J Zucali for advice in establishing the CFU assays and Ms. Rose Mills for secretarial help. This work was supported by NIH Grants CA-09126, Al-1796, and HD-13794.",

year = "1988",

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doi = "10.1016/0042-6822(88)90491-6",

language = "English (US)",

volume = "162",

pages = "483--486",

journal = "Virology",

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T1 - Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector

AU - Laface, Drake

AU - Hermonat, Paul

AU - Wakeland, Edward

AU - Peck, Ammon

N1 - Funding Information: We thank Dr. J Zucali for advice in establishing the CFU assays and Ms. Rose Mills for secretarial help. This work was supported by NIH Grants CA-09126, Al-1796, and HD-13794.

PY - 1988/2

Y1 - 1988/2

N2 - We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formation in the presence of geneticin sulfate (G-418). The value of AAV as an alternative to the retrovirus vector systems is discussed.

AB - We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formation in the presence of geneticin sulfate (G-418). The value of AAV as an alternative to the retrovirus vector systems is discussed.

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JF - Virology

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ER -

Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector

Abstract

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