Generating knockout rats by transposon mutagenesis in spermatogonial stem cells

Zsuzsanna Izsvák, Janine Fröhlich, Ivana Grabundzija, James R. Shirley, Heather M. Powell, Karen M. Chapman, Zoltán Ivics, F. Kent Hamra

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

Disrupting genes in the rat on a genome-wide scale will allow the investigation of many biological processes linked to human health. Here we used transposon-mediated mutagenesis to knock out genes in rat spermatogonial stem cells. Given the capacity of the testis to support spermatogenesis from thousands of transplanted, genetically manipulated spermatogonia, this approach paves a way for high-throughput functional genomic studies in the laboratory rat.

Original languageEnglish (US)
Pages (from-to)443-445
Number of pages3
JournalNature Methods
Volume7
Issue number6
DOIs
Publication statusPublished - Jun 2010

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ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Izsvák, Z., Fröhlich, J., Grabundzija, I., Shirley, J. R., Powell, H. M., Chapman, K. M., ... Hamra, F. K. (2010). Generating knockout rats by transposon mutagenesis in spermatogonial stem cells. Nature Methods, 7(6), 443-445. https://doi.org/10.1038/nmeth.1461