Generating knockout rats by transposon mutagenesis in spermatogonial stem cells

Zsuzsanna Izsvák, Janine Fröhlich, Ivana Grabundzija, James R. Shirley, Heather M. Powell, Karen M. Chapman, Zoltán Ivics, F. Kent Hamra

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

Disrupting genes in the rat on a genome-wide scale will allow the investigation of many biological processes linked to human health. Here we used transposon-mediated mutagenesis to knock out genes in rat spermatogonial stem cells. Given the capacity of the testis to support spermatogenesis from thousands of transplanted, genetically manipulated spermatogonia, this approach paves a way for high-throughput functional genomic studies in the laboratory rat.

Original languageEnglish (US)
Pages (from-to)443-445
Number of pages3
JournalNature methods
Volume7
Issue number6
DOIs
StatePublished - Jun 1 2010

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Izsvák, Z., Fröhlich, J., Grabundzija, I., Shirley, J. R., Powell, H. M., Chapman, K. M., Ivics, Z., & Hamra, F. K. (2010). Generating knockout rats by transposon mutagenesis in spermatogonial stem cells. Nature methods, 7(6), 443-445. https://doi.org/10.1038/nmeth.1461