Generation and characterization of an antagonistic monoclonal antibody against an extracellular domain of mouse DP2 (CRTH2/ GPR44) receptors for prostaglandin D2

Nanae Nagata, Hiroko Iwanari, Hidetoshi Kumagai, Osamu Kusano-Arai, Yuichi Ikeda, Kosuke Aritake, Takao Hamakubo, Yoshihiro Urade

Research output: Contribution to journalArticle

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Abstract

Prostaglandin D2 (PGD2 ) is a lipid mediator involved in sleep regulation and inflammation. PGD2 interacts with 2 types of G protein-coupled receptors, DP1 and DP2/CRTH2 (chemoattractant receptor homologous molecule expressed on T helper type 2 cells)/GPR44 to show a variety of biological effects. DP1 activation leads to Gs-mediated elevation of the intracellular cAMP level, whereas activation of DP2 decreases this level via the Gi pathway; and it also induces G protein-independent, arrestin-mediated cellular responses. Activation of DP2 by PGD2 causes the progression of inflammation via the recruitment of lymphocytes by enhancing the production of Th2-cytokines. Here we developed monoclonal antibodies (MAbs) against the extracellular domain of mouse DP2 by immunization of DP2-null mutant mice with DP2-overexpressing BAF3, murine interleukin-3 dependent pro-B cells, to reduce the generation of antibodies against the host cells by immunization of mice. Moreover, we immunized DP2-KO mice to prevent immunological tolerance to mDP2 protein. After cell ELISA, immunocytochemical, and Western blot analyses, we successfully obtained a novel monoclonal antibody, MAb-1D8, that specifically recognized native mouse DP2, but neither human DP2 nor denatured mouse DP2, by binding to a particular 3D receptor conformation formed by the N-terminus and extracellular loop 1, 2, and 3 of DP2. This antibody inhibited the binding of 0.5 nM [3 H]PGD2 to mouse DP2 (IC50 = 46.3 ± 18.6 nM), showed antagonistic activity toward 15(R)-15-methyl PGD2 -induced inhibition of 300 nM forskolin-activated cAMP production (IC50 = 16.9 ± 2.6 nM), and gave positive results for immunohistochemical staining of DP2-expressing CD4+ Th2 lymphocytes that had accumulated in the kidney of unilateral ureteral obstruction model mice. This monoclonal antibody will be very useful for in vitro and in vivo studies on DP2-mediated diseases.

Original languageEnglish (US)
Article numbere0175452
JournalPLoS One
Volume12
Issue number4
DOIs
StatePublished - Apr 1 2017

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Prostaglandin D2
prostaglandins
monoclonal antibodies
Monoclonal Antibodies
receptors
mice
Immunization
Lymphocytes
Chemical activation
CCR10 Receptors
Formyl Peptide Receptor
Arrestin
Antibodies
Interleukin-3
Inhibitory Concentration 50
inhibitory concentration 50
Colforsin
immunization
lymphocytes
GTP-Binding Proteins

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Generation and characterization of an antagonistic monoclonal antibody against an extracellular domain of mouse DP2 (CRTH2/ GPR44) receptors for prostaglandin D2. / Nagata, Nanae; Iwanari, Hiroko; Kumagai, Hidetoshi; Kusano-Arai, Osamu; Ikeda, Yuichi; Aritake, Kosuke; Hamakubo, Takao; Urade, Yoshihiro.

In: PLoS One, Vol. 12, No. 4, e0175452, 01.04.2017.

Research output: Contribution to journalArticle

Nagata, Nanae ; Iwanari, Hiroko ; Kumagai, Hidetoshi ; Kusano-Arai, Osamu ; Ikeda, Yuichi ; Aritake, Kosuke ; Hamakubo, Takao ; Urade, Yoshihiro. / Generation and characterization of an antagonistic monoclonal antibody against an extracellular domain of mouse DP2 (CRTH2/ GPR44) receptors for prostaglandin D2. In: PLoS One. 2017 ; Vol. 12, No. 4.
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AU - Kumagai, Hidetoshi

AU - Kusano-Arai, Osamu

AU - Ikeda, Yuichi

AU - Aritake, Kosuke

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AU - Urade, Yoshihiro

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