Generation and characterization of KsprtTA and KsptTA transgenic mice

Xinchao Pan, Erin V. Small, Peter Igarashi, Thomas J. Carroll

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The advent of technologies that allow tissue specific expression or ablation of genes has contributed enormously to our knowledge of the mechanism regulating organ development and maintenance in mice. The tetracycline inducible system allows reversible regulation of gene products upon administration of doxycycline. Here we describe the generation and activity of two transgenic lines expressing the cDNAs for the Tet responsive transcription factors rtTA and tTA (Tet-on and off) respectively under the control of an element that drives expression in the epithelium of the developing and adult kidney. Both lines show inducible and reversible activity in the embryonic and adult organ.

Original languageEnglish (US)
Pages (from-to)430-435
Number of pages6
JournalGenesis
Volume51
Issue number6
DOIs
StatePublished - Jun 2013

Fingerprint

Transgenic Mice
Doxycycline
Tetracycline
Genes
Transcription Factors
Epithelium
Complementary DNA
Maintenance
Technology
Kidney

Keywords

  • Kidney development
  • Kidney epithelium
  • Kidney tubule
  • Tet-off
  • Tet-on

ASJC Scopus subject areas

  • Endocrinology
  • Cell Biology
  • Genetics
  • Medicine(all)

Cite this

Generation and characterization of KsprtTA and KsptTA transgenic mice. / Pan, Xinchao; Small, Erin V.; Igarashi, Peter; Carroll, Thomas J.

In: Genesis, Vol. 51, No. 6, 06.2013, p. 430-435.

Research output: Contribution to journalArticle

Pan, Xinchao ; Small, Erin V. ; Igarashi, Peter ; Carroll, Thomas J. / Generation and characterization of KsprtTA and KsptTA transgenic mice. In: Genesis. 2013 ; Vol. 51, No. 6. pp. 430-435.
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