Generation of a hybrid sequence-specific single-stranded deoxyribonuclease

D. R. Corey, P. G. Schultz

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

The relatively nonspecific single-stranded deoxyribonuclease, staphylococcal nuclease, was selectively fused to an oligonucleotide binding site of defined sequence to generate a hybrid enzyme. A cysteine was substituted for Lys116 in the enzyme by oligonucleotide-directed mutagenesis and coupled to an oligonucleotide that contained a 3′-thiol. The resulting hybrid enzyme cleaved single-stranded DNA at sites adjacent to the oligonucleotide binding site.

Original languageEnglish (US)
Pages (from-to)1401-1403
Number of pages3
JournalScience
Volume238
Issue number4832
StatePublished - 1987

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Deoxyribonucleases
Oligonucleotides
Enzymes
Binding Sites
Micrococcal Nuclease
Single-Stranded DNA
Site-Directed Mutagenesis
Sulfhydryl Compounds
Cysteine

ASJC Scopus subject areas

  • General

Cite this

Generation of a hybrid sequence-specific single-stranded deoxyribonuclease. / Corey, D. R.; Schultz, P. G.

In: Science, Vol. 238, No. 4832, 1987, p. 1401-1403.

Research output: Contribution to journalArticle

Corey, DR & Schultz, PG 1987, 'Generation of a hybrid sequence-specific single-stranded deoxyribonuclease', Science, vol. 238, no. 4832, pp. 1401-1403.
Corey, D. R. ; Schultz, P. G. / Generation of a hybrid sequence-specific single-stranded deoxyribonuclease. In: Science. 1987 ; Vol. 238, No. 4832. pp. 1401-1403.
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