Generation of a hybrid sequence-specific single-stranded deoxyribonuclease

D. R. Corey, P. G. Schultz

Research output: Contribution to journalArticle

128 Scopus citations

Abstract

The relatively nonspecific single-stranded deoxyribonuclease, staphylococcal nuclease, was selectively fused to an oligonucleotide binding site of defined sequence to generate a hybrid enzyme. A cysteine was substituted for Lys116 in the enzyme by oligonucleotide-directed mutagenesis and coupled to an oligonucleotide that contained a 3′-thiol. The resulting hybrid enzyme cleaved single-stranded DNA at sites adjacent to the oligonucleotide binding site.

Original languageEnglish (US)
Pages (from-to)1401-1403
Number of pages3
JournalScience
Volume238
Issue number4832
DOIs
StatePublished - Jan 1 1987

ASJC Scopus subject areas

  • General

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