Generation of BAF53b-Cre transgenic mice with pan-neuronal Cre activities

Xiaoming Zhan, Mou Cao, Andrew S. Yoo, Zilai Zhang, Lei Chen, Gerald R. Crabtree, Jiang I. Wu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Molecular and functional studies of genes in neurons in mouse models require neuron-specific Cre lines. The current available neuronal Cre transgenic or knock-in lines either result in expression in a subset of neurons or expression in both neuronal and non-neuronal tissues. Previously we identified BAF53b as a neuron-specific subunit of the chromatin remodeling BAF complexes. Using a bacteria artificial chromosome (BAC) construct containing the BAF53b gene, we generated a Cre transgenic mouse under the control of BAF53b regulatory elements. Like the endogenous BAF53b gene, we showed that BAF53b-Cre is largely neuron-specific. In both central and peripheral nervous systems, it was expressed in all developing neurons examined and was not observed in neural progenitors or glial cells. In addition, BAF53b-Cre functioned in primary cultures in a pan-neuron-specific manner. Thus, BAF53b-Cre mice will be a useful genetic tool to manipulate gene expression in developing neurons for molecular, biochemical, and functional studies.

Original languageEnglish (US)
Pages (from-to)440-448
Number of pages9
JournalGenesis
Volume53
Issue number7
DOIs
StatePublished - Jul 1 2015

Fingerprint

Transgenic Mice
Neurons
Artificial Chromosomes
Genes
Chromatin Assembly and Disassembly
Peripheral Nervous System
Neuroglia
Stem Cells
Central Nervous System
Bacteria
Gene Expression

Keywords

  • Genetics
  • Mammal
  • Organism

ASJC Scopus subject areas

  • Endocrinology
  • Cell Biology
  • Genetics

Cite this

Generation of BAF53b-Cre transgenic mice with pan-neuronal Cre activities. / Zhan, Xiaoming; Cao, Mou; Yoo, Andrew S.; Zhang, Zilai; Chen, Lei; Crabtree, Gerald R.; Wu, Jiang I.

In: Genesis, Vol. 53, No. 7, 01.07.2015, p. 440-448.

Research output: Contribution to journalArticle

Zhan, X, Cao, M, Yoo, AS, Zhang, Z, Chen, L, Crabtree, GR & Wu, JI 2015, 'Generation of BAF53b-Cre transgenic mice with pan-neuronal Cre activities', Genesis, vol. 53, no. 7, pp. 440-448. https://doi.org/10.1002/dvg.22866
Zhan X, Cao M, Yoo AS, Zhang Z, Chen L, Crabtree GR et al. Generation of BAF53b-Cre transgenic mice with pan-neuronal Cre activities. Genesis. 2015 Jul 1;53(7):440-448. https://doi.org/10.1002/dvg.22866
Zhan, Xiaoming ; Cao, Mou ; Yoo, Andrew S. ; Zhang, Zilai ; Chen, Lei ; Crabtree, Gerald R. ; Wu, Jiang I. / Generation of BAF53b-Cre transgenic mice with pan-neuronal Cre activities. In: Genesis. 2015 ; Vol. 53, No. 7. pp. 440-448.
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