TY - JOUR
T1 - Generation of transgenic mice from yeast artificial chromosome DNA that has been modified by gene targeting
AU - McCormick, Sally P A
AU - Peterson, Kenneth R.
AU - Hammer, Robert E
AU - Clegg, Christopher H.
AU - Young, Stephen G.
N1 - Funding Information:
This work was supported by National Institutes of Health (NIH) grants HL 41633, HL 20899, DK30852 and DK 45635. S.P.A.M. is the recipient of a fellowship award from the California Affiliate of the American Heart Association.
PY - 1996/1
Y1 - 1996/1
N2 - Yeast artificial chromosome (YAC) vectors permit the cloning of up to megabase fragments of human genomic DNA in yeast (Saccharomyces cerevisiae). Efficient recombination between homologous segments of DNA is one of the hallmark genetic features of yeast. This characteristic facilitates the introduction of specific mutations into YACs by gene targeting. Gene targeting has been used recently to introduce specific mutations into YACs spanning the human β-globin locus and the human apolipoprotein (apo)-B gene, and the mutated YAC DNA has been used to generate transgenic mice. Tiffs approach has been useful for the study of the regulatory elements controlling β-globin gene expression and/or the study of apo-B structure and function. This article reviews the techniques for introducing mutations into YACs, for the purpose of expression in transgenic mice.
AB - Yeast artificial chromosome (YAC) vectors permit the cloning of up to megabase fragments of human genomic DNA in yeast (Saccharomyces cerevisiae). Efficient recombination between homologous segments of DNA is one of the hallmark genetic features of yeast. This characteristic facilitates the introduction of specific mutations into YACs by gene targeting. Gene targeting has been used recently to introduce specific mutations into YACs spanning the human β-globin locus and the human apolipoprotein (apo)-B gene, and the mutated YAC DNA has been used to generate transgenic mice. Tiffs approach has been useful for the study of the regulatory elements controlling β-globin gene expression and/or the study of apo-B structure and function. This article reviews the techniques for introducing mutations into YACs, for the purpose of expression in transgenic mice.
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U2 - 10.1016/1050-1738(95)00125-5
DO - 10.1016/1050-1738(95)00125-5
M3 - Short survey
C2 - 21232270
AN - SCOPUS:0030064273
SN - 1050-1738
VL - 6
SP - 16
EP - 24
JO - Trends in Cardiovascular Medicine
JF - Trends in Cardiovascular Medicine
IS - 1
ER -