Generation of ultra-small PLGA nanoparticles by sequential centrifugation

Xingwang Wu, Jiangbing Zhou, Toral R. Patel

Research output: Chapter in Book/Report/Conference proceedingChapter


Direct, local delivery of polymer nanoparticles to the brain is a promising strategy to bypass the blood-brain barrier (BBB) and safely deliver a large therapeutic payload. However, even with the aid of convection-enhanced delivery (CED) techniques, this approach has been limited by the inability to fabricate appropriately sized polymer nanoparticles. Here, we outline a versatile and efficient method for producing polymer nanoparticles that are <100 nm in diameter and can be delivered to the brain via CED.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages8
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745



  • Blood-brain barrier
  • Brain
  • Convection-enhanced delivery
  • Nanoparticle
  • PLGA
  • Polymer

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Wu, X., Zhou, J., & Patel, T. R. (2018). Generation of ultra-small PLGA nanoparticles by sequential centrifugation. In Methods in Molecular Biology (pp. 17-24). (Methods in Molecular Biology; Vol. 1831). Humana Press Inc..