Genes for Tfb2, Tfb3, and Tfb4 subunits of yeast transcription/repair factor IIH: Homology to human cyclin-dependent kinase activating kinase and iih subunits

William John Feaver, N. Lynn Henry, Zhigang Wang, Xiaohua Wu, Jesper Q. Svejstrup, David A. Bushnell, Errol C. Friedberg, Roger D. Kornberg

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Genes for the Tfb2, Tfb3, and Tfb4 subunits of yeast RNA polymerase transcription factor IIH (TFIIH) are described. All three genes are essential for cell viability, and antibodies against Tfb3 specifically inhibit transcription in vitro. A C-terminal deletion of Tfb2 caused a defect in nucleotide excision repair, as shown by UV sensitivity of the mutant strain and loss of nucleotide excision repair activity in cell extracts (restored by the addition of purified TFIIH). An interaction between Tfb3 and the Kin28 subunit of TFIIH was detected by the two-hybrid approach, consistent with a role for Tfb3 in linking kinase and core domains of the factor. The deduced amino acid sequence of Tfb2 is similar to that of the 52-kDa subunit of human TFIIH, while Tfb3 is identified as a RING finger protein homologous to the 36kDa subunit of murine CAK (cyclin-dependent kinase activating kinase) and to the 32-kDa subunit of human TFIIH. Tfb4 is homologous to p34 of human TFIIH and is identified as the weakly associated 37-kDa subunit of the yeast factor. These and other findings reveal a one-to-one correspondence and high degree of sequence similarity between the entire set of yeast and human TFIIH polypeptides.

Original languageEnglish (US)
Pages (from-to)19319-19327
Number of pages9
JournalJournal of Biological Chemistry
Volume272
Issue number31
DOIs
StatePublished - Aug 1 1997

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Transcription Factor TFIIH
Transcription
Yeast
Repair
Genes
Yeasts
DNA Repair
Nucleotides
Essential Genes
DNA-Directed RNA Polymerases
cyclin-dependent kinase-activating kinase
Cell Extracts
Amino Acid Sequence
Cell Survival
Phosphotransferases
Cells
Amino Acids
Defects
Peptides
Antibodies

ASJC Scopus subject areas

  • Biochemistry

Cite this

Genes for Tfb2, Tfb3, and Tfb4 subunits of yeast transcription/repair factor IIH : Homology to human cyclin-dependent kinase activating kinase and iih subunits. / Feaver, William John; Henry, N. Lynn; Wang, Zhigang; Wu, Xiaohua; Svejstrup, Jesper Q.; Bushnell, David A.; Friedberg, Errol C.; Kornberg, Roger D.

In: Journal of Biological Chemistry, Vol. 272, No. 31, 01.08.1997, p. 19319-19327.

Research output: Contribution to journalArticle

Feaver, William John ; Henry, N. Lynn ; Wang, Zhigang ; Wu, Xiaohua ; Svejstrup, Jesper Q. ; Bushnell, David A. ; Friedberg, Errol C. ; Kornberg, Roger D. / Genes for Tfb2, Tfb3, and Tfb4 subunits of yeast transcription/repair factor IIH : Homology to human cyclin-dependent kinase activating kinase and iih subunits. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 31. pp. 19319-19327.
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abstract = "Genes for the Tfb2, Tfb3, and Tfb4 subunits of yeast RNA polymerase transcription factor IIH (TFIIH) are described. All three genes are essential for cell viability, and antibodies against Tfb3 specifically inhibit transcription in vitro. A C-terminal deletion of Tfb2 caused a defect in nucleotide excision repair, as shown by UV sensitivity of the mutant strain and loss of nucleotide excision repair activity in cell extracts (restored by the addition of purified TFIIH). An interaction between Tfb3 and the Kin28 subunit of TFIIH was detected by the two-hybrid approach, consistent with a role for Tfb3 in linking kinase and core domains of the factor. The deduced amino acid sequence of Tfb2 is similar to that of the 52-kDa subunit of human TFIIH, while Tfb3 is identified as a RING finger protein homologous to the 36kDa subunit of murine CAK (cyclin-dependent kinase activating kinase) and to the 32-kDa subunit of human TFIIH. Tfb4 is homologous to p34 of human TFIIH and is identified as the weakly associated 37-kDa subunit of the yeast factor. These and other findings reveal a one-to-one correspondence and high degree of sequence similarity between the entire set of yeast and human TFIIH polypeptides.",
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