Genetic analysis of the human type-1 angiotensin II receptor

Kathleen M. Curnow, Leigh Pascoe, Perrin C. White

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

Angiotensin II is a potent pressor hormone and a primary regulator of aldosterone secretion. It acts through at least two types of receptors termed AT1 and AT2. We analyzed cDNA and genomic clones encoding the human angiotensin II type-1 receptor, AT1. The human AT1 gene was mapped to chromosome 3q by polymerase chain reaction analysis of DNA from a panel of human-hamster somatic cell hybrids. The predicted amino acid sequence is 95% identical to the corresponding rat and bovine receptors and 25% and 22% identical, respectively, to the receptors encoded by the RTA and MAS genes. Characterization of several human cDNA clones demonstrated the existence of two alternate 5′-untranslated regions (UTRs) that contain a common initial sequence but differ by the presence or absence of an insertion of 84 base pairs. In the genomic sequence, the coding sequences are contained in a single exon, with an intron occurring in the 5′-UTR at the position of insertion of the 84-base pair sequence. The exons encoding the alternate 5′-UTRs are located at least 3.8 kilobases away from the exon encoding the protein. Reverse transcription-polymerase chain reaction analysis showed that both forms of 5′-UTR are present in approximately equal abundance in a range of tissues expressing AT1. The reagents developed in this work may be useful in testing the hypothesis that genetic variations in angiotensin II receptor function are associated with a tendency to develop hypertension.

Original languageEnglish (US)
Pages (from-to)1113-1118
Number of pages6
JournalMolecular Endocrinology
Volume6
Issue number7
DOIs
StatePublished - Jul 1 1992

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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