Genetic analysis of the human type-1 angiotensin II receptor

Kathleen M. Curnow, Leigh Pascoe, Perrin C. White

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Angiotensin II is a potent pressor hormone and a primary regulator of aldosterone secretion. It acts through at least two types of receptors termed AT1 and AT2. We analyzed cDNA and genomic clones encoding the human angiotensin II type-1 receptor, AT1. The human AT1 gene was mapped to chromosome 3q by polymerase chain reaction analysis of DNA from a panel of human-hamster somatic cell hybrids. The predicted amino acid sequence is 95% identical to the corresponding rat and bovine receptors and 25% and 22% identical, respectively, to the receptors encoded by the RTA and MAS genes. Characterization of several human cDNA clones demonstrated the existence of two alternate 5′-untranslated regions (UTRs) that contain a common initial sequence but differ by the presence or absence of an insertion of 84 base pairs. In the genomic sequence, the coding sequences are contained in a single exon, with an intron occurring in the 5′-UTR at the position of insertion of the 84-base pair sequence. The exons encoding the alternate 5′-UTRs are located at least 3.8 kilobases away from the exon encoding the protein. Reverse transcription-polymerase chain reaction analysis showed that both forms of 5′-UTR are present in approximately equal abundance in a range of tissues expressing AT1. The reagents developed in this work may be useful in testing the hypothesis that genetic variations in angiotensin II receptor function are associated with a tendency to develop hypertension.

Original languageEnglish (US)
Pages (from-to)1113-1118
Number of pages6
JournalMolecular Endocrinology
Volume6
Issue number7
StatePublished - Jul 1992

Fingerprint

Angiotensin Type 1 Receptor
5' Untranslated Regions
Medical Genetics
Exons
Base Pairing
Complementary DNA
Clone Cells
Polymerase Chain Reaction
Angiotensin Receptors
Hybrid Cells
Aldosterone
Angiotensin II
Cricetinae
Introns
Genes
Reverse Transcription
Amino Acid Sequence
Chromosomes
Hormones
Hypertension

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Genetic analysis of the human type-1 angiotensin II receptor. / Curnow, Kathleen M.; Pascoe, Leigh; White, Perrin C.

In: Molecular Endocrinology, Vol. 6, No. 7, 07.1992, p. 1113-1118.

Research output: Contribution to journalArticle

Curnow, Kathleen M. ; Pascoe, Leigh ; White, Perrin C. / Genetic analysis of the human type-1 angiotensin II receptor. In: Molecular Endocrinology. 1992 ; Vol. 6, No. 7. pp. 1113-1118.
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