Genetic analysis of the role of the Drosophila fat facets gene in the ubiquitin pathway

Zhuoru Wu, Qinghong Li, Mark E. Fortini, Janice A. Fischer

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The Drosophila fat facets gene encodes a deubiquitinating enzyme required during eye development to limit the number of photoreceptors in each facet to eight. Ubiquitin is a small polypeptide that targets proteins for degradation by the proteasome. Deubiquitinating enzymes cleave ubiquitin- protein bonds. In order to investigate the role of FAT FACETS in the ubiquitin pathway, genetic interactions between fat facets and the Drosophila UbcD 1 gene were assessed. In addition, three yeast deubiquitinating enzyme genes were tested for their ability to substitute for fat facets in the developing Drosophila eye and for their effects on eye morphology. The results of these experiments support the hypothesis that FAT FACETS activity antagonizes that of the proteolytic machinery. The implications of these results for the specificity of FAF and yeast UBPs are discussed as well.

Original languageEnglish (US)
Pages (from-to)312-320
Number of pages9
JournalDevelopmental Genetics
Volume25
Issue number4
StatePublished - 1999

Fingerprint

Ubiquitin
Drosophila
Fats
Fat Substitutes
Yeasts
Genes
Proteasome Endopeptidase Complex
Proteolysis
Peptides
Deubiquitinating Enzymes
Proteins

Keywords

  • Deubiquitinating enzyme
  • Drosophila
  • Eye development
  • Fat facets
  • Ubiquitin
  • UBP

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics

Cite this

Wu, Z., Li, Q., Fortini, M. E., & Fischer, J. A. (1999). Genetic analysis of the role of the Drosophila fat facets gene in the ubiquitin pathway. Developmental Genetics, 25(4), 312-320.

Genetic analysis of the role of the Drosophila fat facets gene in the ubiquitin pathway. / Wu, Zhuoru; Li, Qinghong; Fortini, Mark E.; Fischer, Janice A.

In: Developmental Genetics, Vol. 25, No. 4, 1999, p. 312-320.

Research output: Contribution to journalArticle

Wu, Z, Li, Q, Fortini, ME & Fischer, JA 1999, 'Genetic analysis of the role of the Drosophila fat facets gene in the ubiquitin pathway', Developmental Genetics, vol. 25, no. 4, pp. 312-320.
Wu, Zhuoru ; Li, Qinghong ; Fortini, Mark E. ; Fischer, Janice A. / Genetic analysis of the role of the Drosophila fat facets gene in the ubiquitin pathway. In: Developmental Genetics. 1999 ; Vol. 25, No. 4. pp. 312-320.
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