Genetic and mechanistic analyses of the periplasmic domain of the enterohemorrhagic Escherichia coli QseC histidine sensor kinase

Christopher T. Parker, Regan Russell, Jacqueline W. Njoroge, Angel G. Jimenez, Ron Taussig, Vanessa Sperandio

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The histidine sensor kinase (HK) QseC senses autoinducer 3 (AI-3) and the adrenergic hormones epinephrine and norepinephrine. Upon sensing these signals, QseC acts through three response regulators (RRs) to regulate the expression of virulence genes in enterohemorrhagic Escherichia coli (EHEC). The QseB, QseF, and KdpE RRs that are phosphorylated by QseC constitute a tripartite signaling cascade having different and overlapping targets, including flagella and motility, the type three secretion system encoded by the locus of enterocyte effacement (LEE), and Shiga toxin. We modeled the tertiary structure of QseC's periplasmic sensing domain and aligned the sequences from 12 different species to identify the most conserved amino acids. We selected eight amino acids conserved in all of these QseC homologues. The corresponding QseC site-directed mutants were expressed and still able to autophosphorylate; however, four mutants demonstrated an increased basal level of phosphorylation. These mutants have differential flagellar, motility, LEE, and Shiga toxin expression phenotypes. We selected four mutants for more in-depth analyses and found that they differed in their ability to phosphorylate QseB, KdpE, and QseF. This suggests that these mutations in the periplasmic sensing domain affected the region downstream of the QseC signaling cascade and therefore can influence which pathway QseC regulates.

Original languageEnglish (US)
Article numbere00861-16
JournalJournal of Bacteriology
Volume199
Issue number8
DOIs
StatePublished - Apr 1 2017

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Shiga Toxin
Enterohemorrhagic Escherichia coli
Enterocytes
Amino Acids
Flagella
Adrenergic Agents
Epinephrine
Virulence
Norepinephrine
Phosphorylation
Hormones
Phenotype
Gene Expression
Mutation
Histidine Kinase

Keywords

  • EHEC
  • Enterohemorrhagic E. coli
  • LEE
  • QseC
  • Two-component system

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Genetic and mechanistic analyses of the periplasmic domain of the enterohemorrhagic Escherichia coli QseC histidine sensor kinase. / Parker, Christopher T.; Russell, Regan; Njoroge, Jacqueline W.; Jimenez, Angel G.; Taussig, Ron; Sperandio, Vanessa.

In: Journal of Bacteriology, Vol. 199, No. 8, e00861-16, 01.04.2017.

Research output: Contribution to journalArticle

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