Genetic and molecular characterization of tube, a Drosophila gene maternally required for embryonic dorsoventral polarity

Anthea Letsou, Sherry Alexander, Kim Orth, Steven A. Wasserman

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Loss of maternal function of the tube gene disrupts a signaling pathway required for pattern formation in Drosophila, causing cells throughout the embryo to adopt the fate normally reserved for those at the dorsal surface. Here we demonstrate that tube mutations also have a zygotic effect on pupal morphology and that this phenotype is shared by mutations in Toll and pelle, two genes with apparent intracellular roles in determining dorsoventral polarity. We then describe the isolation of a functionally full-length tube cDNA identified in a phenotypic rescue assay. The tube mRNA is expressed maximally early in embryogenesis and again late in larval development, corresponding to required periods of tube activity as defined by distinct maternal and zygotic loss-of-function phenotypes in tube mutants. Sequence analysis of the cDNA indicates that the tube protein contains five copies of an eight-residue motif and shares no significant sequence similarity with known proteins. These results suggest that tube represents a class of protein active in signal transduction at two stages of development.

Original languageEnglish (US)
Pages (from-to)810-814
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number3
StatePublished - 1991

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Drosophila
Molecular Biology
Complementary DNA
Mothers
Genes
Phenotype
Mutation
Proteins
Embryonic Development
Sequence Analysis
Signal Transduction
Embryonic Structures
Messenger RNA

Keywords

  • Pattern formation
  • Pelle
  • Signal transduction
  • Toll

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Genetic and molecular characterization of tube, a Drosophila gene maternally required for embryonic dorsoventral polarity. / Letsou, Anthea; Alexander, Sherry; Orth, Kim; Wasserman, Steven A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 3, 1991, p. 810-814.

Research output: Contribution to journalArticle

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