TY - JOUR
T1 - Genetic basis for altered pathogenesis of an immune-selected antigenic variant of reovirus type 3 (Dearing)
AU - Kaye, K. M.
AU - Spriggs, D. R.
AU - Bassel-Duby, R.
AU - Fields, B. N.
AU - Tyler, K. L.
PY - 1986
Y1 - 1986
N2 - In this paper we provide a step by step comparison of the pathogenesis of murine infection caused by reovirus type 3 (Dearing) and an antigenic variant (K) selected by its resistance to neutralization with a monoclonal antibody (G5) directed against the T3 hemagglutinin. To show that specific changes in the biiologic properties of variant K were due to mutation in the S1 double-stranded RNA segment (gene), which encodes the viral hemagglutinin, we generated a reassortant virus ('1 HA K') containing the variant K S1 gene and compared its properties to variant K and to a reassortant (1 HA 3') containing T3 (Dearing) S1 gene. These studies, in conjunction with our previous nucleotide sequence analysis of the S1 genes of variant K and T3 (Dearing) [R. Bassel-Duby, A. Jayasuriya, D.Chatterjee, N. Sonenberg, J.V. Maizel, Jr., and B.N. Fields, Nature (London) 315:421-423, 1985; R. Bassel-Duby, D.R. Spriggs, K.L. Tyler, and B.N. Fields, submitted for publication], indicate that a single amino acid change in the T3 hemagglutinin can alter viral growth and tropism within the central nervous system without affecting either its primary replication in the intestine or its pattern of spread to or within the central nervous system.
AB - In this paper we provide a step by step comparison of the pathogenesis of murine infection caused by reovirus type 3 (Dearing) and an antigenic variant (K) selected by its resistance to neutralization with a monoclonal antibody (G5) directed against the T3 hemagglutinin. To show that specific changes in the biiologic properties of variant K were due to mutation in the S1 double-stranded RNA segment (gene), which encodes the viral hemagglutinin, we generated a reassortant virus ('1 HA K') containing the variant K S1 gene and compared its properties to variant K and to a reassortant (1 HA 3') containing T3 (Dearing) S1 gene. These studies, in conjunction with our previous nucleotide sequence analysis of the S1 genes of variant K and T3 (Dearing) [R. Bassel-Duby, A. Jayasuriya, D.Chatterjee, N. Sonenberg, J.V. Maizel, Jr., and B.N. Fields, Nature (London) 315:421-423, 1985; R. Bassel-Duby, D.R. Spriggs, K.L. Tyler, and B.N. Fields, submitted for publication], indicate that a single amino acid change in the T3 hemagglutinin can alter viral growth and tropism within the central nervous system without affecting either its primary replication in the intestine or its pattern of spread to or within the central nervous system.
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U2 - 10.1128/jvi.59.1.90-97.1986
DO - 10.1128/jvi.59.1.90-97.1986
M3 - Article
C2 - 3012122
AN - SCOPUS:0022491811
SN - 0022-538X
VL - 59
SP - 90
EP - 97
JO - Journal of virology
JF - Journal of virology
IS - 1
ER -