Genetic basis for altered pathogenesis of an immune-selected antigenic variant of reovirus type 3 (Dearing)

K. M. Kaye, D. R. Spriggs, R. Bassel-Duby, B. N. Fields, K. L. Tyler

Research output: Contribution to journalArticle

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Abstract

In this paper we provide a step by step comparison of the pathogenesis of murine infection caused by reovirus type 3 (Dearing) and an antigenic variant (K) selected by its resistance to neutralization with a monoclonal antibody (G5) directed against the T3 hemagglutinin. To show that specific changes in the biiologic properties of variant K were due to mutation in the S1 double-stranded RNA segment (gene), which encodes the viral hemagglutinin, we generated a reassortant virus ('1 HA K') containing the variant K S1 gene and compared its properties to variant K and to a reassortant (1 HA 3') containing T3 (Dearing) S1 gene. These studies, in conjunction with our previous nucleotide sequence analysis of the S1 genes of variant K and T3 (Dearing) [R. Bassel-Duby, A. Jayasuriya, D.Chatterjee, N. Sonenberg, J.V. Maizel, Jr., and B.N. Fields, Nature (London) 315:421-423, 1985; R. Bassel-Duby, D.R. Spriggs, K.L. Tyler, and B.N. Fields, submitted for publication], indicate that a single amino acid change in the T3 hemagglutinin can alter viral growth and tropism within the central nervous system without affecting either its primary replication in the intestine or its pattern of spread to or within the central nervous system.

Original languageEnglish (US)
Pages (from-to)90-97
Number of pages8
JournalJournal of Virology
Volume59
Issue number1
StatePublished - 1986

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Mammalian orthoreovirus 3
Reoviridae
triiodothyronine
hemagglutinins
pathogenesis
Hemagglutinins
Genes
central nervous system
genes
Viral Tropism
Central Nervous System
Viral Hemagglutinins
Reassortant Viruses
tropisms
Double-Stranded RNA
double-stranded RNA
neutralization
Intestines
Sequence Analysis
microbial growth

ASJC Scopus subject areas

  • Immunology

Cite this

Genetic basis for altered pathogenesis of an immune-selected antigenic variant of reovirus type 3 (Dearing). / Kaye, K. M.; Spriggs, D. R.; Bassel-Duby, R.; Fields, B. N.; Tyler, K. L.

In: Journal of Virology, Vol. 59, No. 1, 1986, p. 90-97.

Research output: Contribution to journalArticle

Kaye, K. M. ; Spriggs, D. R. ; Bassel-Duby, R. ; Fields, B. N. ; Tyler, K. L. / Genetic basis for altered pathogenesis of an immune-selected antigenic variant of reovirus type 3 (Dearing). In: Journal of Virology. 1986 ; Vol. 59, No. 1. pp. 90-97.
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