Genetic control of resistance to Mycoplasma pulmonis infection in mice

W. C. Lai, G. Linton, M. Bennett, S. P. Pakes

Research output: Contribution to journalArticle

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Abstract

The differences in susceptibility of various inbred strains of mice to a highly pathogenic strain of Mycoplasma pulmonis CT (T2) has been known for some time. We assessed the genetic control of resistance to T2 infection. Tracheolung lavage samples and lungs of mice were assessed for T2 organisms after intratracheal injection of T2. We found that H-2b C57BL/6 (B6) and H- 2(k) B10.BR mice were resistant, whereas H-2b A.By, H-2(k) C3H/Bi, H-2(k) C3H/HeJ (C3H), and H-2b BALB.B mice were susceptible. We also typed individual B6C3F2 mice for H-2 and for resistance to T2 and observed that resistance to T2 infections is controlled by a single dominant gene not linked to H-2. Histologic examination revealed severe lung lesions typical of M. pulmonis infections in susceptible C3H mice, in contrast to minimal lung lesions in resistant B6 mice. No significant titers of local or systemic antimycoplasma antibodies were detected in either resistant or susceptible mice at 5 days postinfection. Macrophages taken from uninfected B6 or C3H mice failed to inhibit growth of T2 in vitro. However, macrophages from B6 mice did inhibit growth of T2 much better than C3H macrophages when harvested on day 5 of infection. Thus, there is an association between activation of macrophage bactericidal function and genetic resistance to growth of T2 organisms.

Original languageEnglish (US)
Pages (from-to)4615-4621
Number of pages7
JournalInfection and Immunity
Volume61
Issue number11
StatePublished - 1993

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Mycoplasma pulmonis
Mycoplasma Infections
Inbred C3H Mouse
Macrophages
Growth
Infection
Dominant Genes
Lung
Inbred Strains Mice
Macrophage Activation
Bronchoalveolar Lavage
Injections
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Lai, W. C., Linton, G., Bennett, M., & Pakes, S. P. (1993). Genetic control of resistance to Mycoplasma pulmonis infection in mice. Infection and Immunity, 61(11), 4615-4621.

Genetic control of resistance to Mycoplasma pulmonis infection in mice. / Lai, W. C.; Linton, G.; Bennett, M.; Pakes, S. P.

In: Infection and Immunity, Vol. 61, No. 11, 1993, p. 4615-4621.

Research output: Contribution to journalArticle

Lai, WC, Linton, G, Bennett, M & Pakes, SP 1993, 'Genetic control of resistance to Mycoplasma pulmonis infection in mice', Infection and Immunity, vol. 61, no. 11, pp. 4615-4621.
Lai WC, Linton G, Bennett M, Pakes SP. Genetic control of resistance to Mycoplasma pulmonis infection in mice. Infection and Immunity. 1993;61(11):4615-4621.
Lai, W. C. ; Linton, G. ; Bennett, M. ; Pakes, S. P. / Genetic control of resistance to Mycoplasma pulmonis infection in mice. In: Infection and Immunity. 1993 ; Vol. 61, No. 11. pp. 4615-4621.
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