Genetic dissection of the miR-17-92 cluster of microRNAs in Myc-induced B-cell lymphomas

Ping Mu, Yoon Chi Han, Doron Betel, Evelyn Yao, Massimo Squatrito, Paul Ogrodowski, Elisa De Stanchina, Aleco D'Andrea, Chris Sander, Andrea Ventura

Research output: Contribution to journalArticle

361 Scopus citations

Abstract

The miR-17-92 cluster is frequently amplified or over-expressed in human cancers and has emerged as the prototypical oncogenic polycistron microRNA (miRNA). miR-17-92 is a direct transcriptional target of c-Myc, and experiments in a mouse model of B-cell lymphomas have shown cooperation between these two oncogenes. However, both the molecular mechanism underlying this cooperation and the individual miRNAs that are responsible for it are unknown. By using a conditional knockout allele of miR-17-92, we show here that sustained expression of endogenous miR-17-92 is required to suppress apoptosis in Myc-driven B-cell lymphomas. Furthermore, we show that among the six miRNAs that are encoded by miR-17-92, miR-19a and miR-19b are absolutely required and largely sufficient to recapitulate the oncogenic properties of the entire cluster. Finally, by combining computational target prediction, gene expression profiling, and an in vitro screening strategy, we identify a subset of miR-19 targets that mediate its prosurvival activity.

Original languageEnglish (US)
Pages (from-to)2806-2811
Number of pages6
JournalGenes and Development
Volume23
Issue number24
DOIs
StatePublished - Dec 15 2009
Externally publishedYes

Keywords

  • Cancer
  • MiR-17-92
  • MicroRNAs
  • Mouse
  • Myc

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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    Mu, P., Han, Y. C., Betel, D., Yao, E., Squatrito, M., Ogrodowski, P., De Stanchina, E., D'Andrea, A., Sander, C., & Ventura, A. (2009). Genetic dissection of the miR-17-92 cluster of microRNAs in Myc-induced B-cell lymphomas. Genes and Development, 23(24), 2806-2811. https://doi.org/10.1101/gad.1872909