Genetic evidence for the vital function of osterix in cementogenesis

Zhengguo Cao, Hua Zhang, Xin Zhou, Xianglong Han, Yinshi Ren, Tian Gao, Yin Xiao, Benoit De Crombrugghe, Martha J. Somerman, Jian Q. Feng

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

To date, attempts to regenerate a complete tooth, including the critical periodontal tissues associated with the tooth root, have not been successful. Controversy still exists regarding the origin of the cell source for cellular cementum (epithelial or mesenchymal). This disagreement may be partially due to a lack of understanding of the events leading to the initiation and development of the tooth roots and supportive tissues, such as the cementum. Osterix (OSX) is a transcriptional factor essential for osteogenesis, but its role in cementogenesis has not been addressed. In the present study, we first documented a close relationship between the temporal- and spatial-expression pattern of Osx and the formation of cellular cementum. We then generated 3.6-kilobase (kb) collagen type I (3.6-kb Col 1)-Osx transgenic mice, which displayed accelerated cementum formation versus wild-type (WT) controls. Importantly, the conditional deletion of Osx in the mesenchymal cells with two different Cre systems (the 2.3-kb Col 1 and an inducible CAG-Cre estrogen receptor [CreER]) led to a sharp reduction in cellular cementum formation (including the cementum mass and mineral deposition rate) and gene expression of dentin matrix protein 1 (DMP1) by cementocytes. However, the deletion of the Osx gene after cellular cementum formed did not alter the properties of the mature cementum as evaluated by backscattered scanning electron microscopy (SEM) and resin-casted SEM. Transient transfection of Osx in the cementoblasts in vitro significantly inhibited cell proliferation and increased cell differentiation and mineralization. Taken together, these data support: (1) the mesenchymal origin of cellular cementum (from periodontal ligament [PDL] progenitor cells); (2) the vital role of OSX in controlling the formation of cellular cementum; and (3) the limited remodeling of cellular cementum in adult mice.

Original languageEnglish (US)
Pages (from-to)1080-1092
Number of pages13
JournalJournal of Bone and Mineral Research
Volume27
Issue number5
DOIs
StatePublished - May 1 2012

Keywords

  • CEMENTOGENESIS
  • CEMENTUM
  • DMP1
  • OSX
  • TOOTH ROOT

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine

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  • Cite this

    Cao, Z., Zhang, H., Zhou, X., Han, X., Ren, Y., Gao, T., Xiao, Y., De Crombrugghe, B., Somerman, M. J., & Feng, J. Q. (2012). Genetic evidence for the vital function of osterix in cementogenesis. Journal of Bone and Mineral Research, 27(5), 1080-1092. https://doi.org/10.1002/jbmr.1552