Genetic fate mapping reveals that the caudal ganglionic eminence produces a large and diverse population of superficial cortical interneurons

Goichi Miyoshi, Jens Hjerling-Leffler, Theofanis Karayannis, Vitor H. Sousa, Simon J B Butt, James Battiste, Jane E. Johnson, Robert P. Machold, Gord Fishell

Research output: Contribution to journalArticle

329 Scopus citations

Abstract

By combining an inducible genetic fate mapping strategy with electrophysiological analysis, we have systematically characterized the populations of cortical GABAergic interneurons that originate from the caudal ganglionic eminence (CGE). Interestingly, compared with medial ganglionic eminence (MGE)-derived cortical interneuron populations, the initiation [embryonic day 12.5 (E12.5)] and peak production (E16.5) of interneurons from this embryonic structure occurs 3 d later in development. Moreover, unlike either pyramidal cells or MGE-derived cortical interneurons, CGE-derived interneurons do not integrate into the cortex in an inside-out manner but preferentially (75%) occupy superficial cortical layers independent of birthdate. In contrast to previous estimates, CGE-derived interneurons are both considerably greater in number (∼30% of all cortical interneurons) and diversity (comprised by at least nine distinct subtypes). Furthermore,wefound that a large proportion of CGE-derived interneurons, including the neurogliaform subtype, express the glycoprotein Reelin. In fact, most CGE-derived cortical interneurons express either Reelin or vasoactive intestinal polypeptide. Thus, in conjunction with previous studies, we have now determined the spatial and temporal origins of the vast majority of cortical interneuron subtypes.

Original languageEnglish (US)
Pages (from-to)1582-1594
Number of pages13
JournalJournal of Neuroscience
Volume30
Issue number5
DOIs
StatePublished - Feb 3 2010

    Fingerprint

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Miyoshi, G., Hjerling-Leffler, J., Karayannis, T., Sousa, V. H., Butt, S. J. B., Battiste, J., Johnson, J. E., Machold, R. P., & Fishell, G. (2010). Genetic fate mapping reveals that the caudal ganglionic eminence produces a large and diverse population of superficial cortical interneurons. Journal of Neuroscience, 30(5), 1582-1594. https://doi.org/10.1523/JNEUROSCI.4515-09.2010