Genetic restrictions of transfer of delayed-type hypersensitivity to sheep erythrocytes: Local versus systemic transfer

Martha Turner Lubet, John R. Kettman

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Regulation of the transfer of delayed-type hypersensitivity (DTH) reactions to SRBC was studied using two assays. In the systemic transfer, SRBC immune cells were transferred intravenously and the recipient challenged by injecting antigen into the footpad. In the local transfer assay, SRBC immune cells were mixed with antigen before transfer into the footpad of the recipient. These studies utilized B10.D2 and B10.BR mice which are congenic strains differing only at H-2 region. DTH reactions can be transferred across H-2 barriers using a local transfer assay. When the immune cells were transferred intravenously or depleted of adherent cells prior to local transfer, DTH reactions cannot be transferred to an H-2 congenic recipient. Spleen cells from naive mice syngeneic to the intravenously transferred cells supply the necessary accessory cell when mixed with the antigen prior to injection into the footpad. This accessory cell may be a macrophage.

Original languageEnglish (US)
Pages (from-to)151-156
Number of pages6
JournalCellular Immunology
Volume45
Issue number1
DOIs
StatePublished - Jun 1979

ASJC Scopus subject areas

  • Immunology

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