Genetic selection of regulatory mutants of mammalian adenylyl cyclases

P. Clapp, A. B. Capper, R. Taussig

Research output: Contribution to journalArticle

Abstract

Initial steps in the identification of the Gsα-binding site present in mammalian adenylyl cyclases can be achieved with the use of the yeast genetic system described. It must be stressed that this system serves as a means to identify mutants that are candidates; biochemical analysis of these mutants is a next and necessary step in the confirmation of these phenotypes. The system described can be readily adapted for the isolation of additional classes of mammalian adenylyl cyclase mutants including mutants with altered regulation toward forskolin, catalytic abnormalities, or enhanced sensitivities toward activators. In addition, this system can be employed for the isolation of constitutively active adenylyl cyclase mutants, or by coexpressing other adenylyl cyclase isoforms and their known regulators, mutations in the binding sites for these molecules can be elucidated.

Original languageEnglish (US)
Pages (from-to)241-251
Number of pages11
JournalMethods in Enzymology
Volume345
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Biochemistry

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