Abstract
As a vertebrate genetic model, the zebrafish has been well recognized for its strength in studying a variety of biological processes and human diseases. Traditional forward genetic screens in zebrafish have generated a large pool of mutants with interesting phenotypes resembling human diseases but the underlying mechanisms are not well understood. A powerful approach to elucidate the mechanisms of these mutants is the modifier screen, which identifies 2 nd-site mutations that specifically enhance or block the phenotype of a given mutant. Here we described the first genetic suppressor screen in zebrafish, which identifies a novel transcriptional mechanism regulating erythropoiesis. In combination with the haploid genetics in zebrafish, we have shown the feasibility and strength of a modifier screen in zebrafish. This strategy will greatly broaden the utility of the zebrafish as a model for making original discoveries and establishing novel paradigms for understanding vertebrate biology.
Original language | English (US) |
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Pages (from-to) | 129-136 |
Number of pages | 8 |
Journal | Methods in Cell Biology |
Volume | 104 |
DOIs | |
State | Published - 2011 |
Keywords
- BAC transgene
- ENU mutagenesis
- Haploid
- Modifier screen
- Suppressor screen
ASJC Scopus subject areas
- Cell Biology