Genetic suppressor screens in haploids

Xiaoying Bai, Zhongan Yang, Hong Jiang, Shuo Lin, Leonard I. Zon

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

As a vertebrate genetic model, the zebrafish has been well recognized for its strength in studying a variety of biological processes and human diseases. Traditional forward genetic screens in zebrafish have generated a large pool of mutants with interesting phenotypes resembling human diseases but the underlying mechanisms are not well understood. A powerful approach to elucidate the mechanisms of these mutants is the modifier screen, which identifies 2 nd-site mutations that specifically enhance or block the phenotype of a given mutant. Here we described the first genetic suppressor screen in zebrafish, which identifies a novel transcriptional mechanism regulating erythropoiesis. In combination with the haploid genetics in zebrafish, we have shown the feasibility and strength of a modifier screen in zebrafish. This strategy will greatly broaden the utility of the zebrafish as a model for making original discoveries and establishing novel paradigms for understanding vertebrate biology.

Original languageEnglish (US)
Pages (from-to)129-136
Number of pages8
JournalMethods in Cell Biology
Volume104
DOIs
StatePublished - 2011

Keywords

  • BAC transgene
  • ENU mutagenesis
  • Haploid
  • Modifier screen
  • Suppressor screen

ASJC Scopus subject areas

  • Cell Biology

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    Bai, X., Yang, Z., Jiang, H., Lin, S., & Zon, L. I. (2011). Genetic suppressor screens in haploids. Methods in Cell Biology, 104, 129-136. https://doi.org/10.1016/B978-0-12-374814-0.00007-0