Genome analysis on linkage group VI of Neurospora crassa

Yihong Wan; Hongjian Liu; Changgong Li; Thomas J. Schmidhauser

doi:10.1006/fgbi.1997.0988

Genome analysis on linkage group VI of Neurospora crassa

Yihong Wan, Hongjian Liu, Changgong Li, Thomas J. Schmidhauser

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6 Scopus citations

Abstract

Two chromosome walks covering 420 and 110 kb on the left arm of linkage group VI (LGVIL) of Neurospora crassa were pursued with the goal of cloning carotenegenic loci. Complementation analysis with clones isolated in the 420-kb walk allowed identification of the yellow-1 (ylo-1) gene which is essential for Neurospora carotenogenesis. We have physically located a second gene, unknown-13 (un-13), between the crosspathway control-1, (cpc-1) and ylo-1 loci. Cloning of a second potential carotenogenic locus, vivid (vvd), from our walks was attempted using screening of Northern blots with radiolabeled DNA fragments from walk clones to identify gene transcripts. The radiolabeled DNA fragments were used to clone complementary DNA isolates representing an additional four genes in the two walks.

Original language	English (US)
Pages (from-to)	329-336
Number of pages	8
Journal	Fungal Genetics and Biology
Volume	21
Issue number	3
DOIs	https://doi.org/10.1006/fgbi.1997.0988
State	Published - Jun 1997

Keywords

Carotenoid
Genome
Neurospora
un-13
ylo-1

ASJC Scopus subject areas

Microbiology
Genetics

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10.1006/fgbi.1997.0988

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title = "Genome analysis on linkage group VI of Neurospora crassa",

abstract = "Two chromosome walks covering 420 and 110 kb on the left arm of linkage group VI (LGVIL) of Neurospora crassa were pursued with the goal of cloning carotenegenic loci. Complementation analysis with clones isolated in the 420-kb walk allowed identification of the yellow-1 (ylo-1) gene which is essential for Neurospora carotenogenesis. We have physically located a second gene, unknown-13 (un-13), between the crosspathway control-1, (cpc-1) and ylo-1 loci. Cloning of a second potential carotenogenic locus, vivid (vvd), from our walks was attempted using screening of Northern blots with radiolabeled DNA fragments from walk clones to identify gene transcripts. The radiolabeled DNA fragments were used to clone complementary DNA isolates representing an additional four genes in the two walks.",

keywords = "Carotenoid, Genome, Neurospora, un-13, ylo-1",

author = "Yihong Wan and Hongjian Liu and Changgong Li and Schmidhauser, {Thomas J.}",

note = "Funding Information: This work was supported by Public Health Services Grant GM-45282 from the National Institutes of Health (to T.J.S.). The authors gratefully acknowledge the support and helpful comments of S. Bennett and D. D. Perkins. Michelle Hall contributed to the identification of un-13+ cosmids. Heather Lucas contributed to the physical mapping of un-13+.",

year = "1997",

month = jun,

doi = "10.1006/fgbi.1997.0988",

language = "English (US)",

volume = "21",

pages = "329--336",

journal = "Fungal Genetics and Biology",

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publisher = "Academic Press Inc.",

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T1 - Genome analysis on linkage group VI of Neurospora crassa

AU - Wan, Yihong

AU - Liu, Hongjian

AU - Li, Changgong

AU - Schmidhauser, Thomas J.

N1 - Funding Information: This work was supported by Public Health Services Grant GM-45282 from the National Institutes of Health (to T.J.S.). The authors gratefully acknowledge the support and helpful comments of S. Bennett and D. D. Perkins. Michelle Hall contributed to the identification of un-13+ cosmids. Heather Lucas contributed to the physical mapping of un-13+.

PY - 1997/6

Y1 - 1997/6

N2 - Two chromosome walks covering 420 and 110 kb on the left arm of linkage group VI (LGVIL) of Neurospora crassa were pursued with the goal of cloning carotenegenic loci. Complementation analysis with clones isolated in the 420-kb walk allowed identification of the yellow-1 (ylo-1) gene which is essential for Neurospora carotenogenesis. We have physically located a second gene, unknown-13 (un-13), between the crosspathway control-1, (cpc-1) and ylo-1 loci. Cloning of a second potential carotenogenic locus, vivid (vvd), from our walks was attempted using screening of Northern blots with radiolabeled DNA fragments from walk clones to identify gene transcripts. The radiolabeled DNA fragments were used to clone complementary DNA isolates representing an additional four genes in the two walks.

AB - Two chromosome walks covering 420 and 110 kb on the left arm of linkage group VI (LGVIL) of Neurospora crassa were pursued with the goal of cloning carotenegenic loci. Complementation analysis with clones isolated in the 420-kb walk allowed identification of the yellow-1 (ylo-1) gene which is essential for Neurospora carotenogenesis. We have physically located a second gene, unknown-13 (un-13), between the crosspathway control-1, (cpc-1) and ylo-1 loci. Cloning of a second potential carotenogenic locus, vivid (vvd), from our walks was attempted using screening of Northern blots with radiolabeled DNA fragments from walk clones to identify gene transcripts. The radiolabeled DNA fragments were used to clone complementary DNA isolates representing an additional four genes in the two walks.

KW - Carotenoid

KW - Genome

KW - Neurospora

KW - un-13

KW - ylo-1

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U2 - 10.1006/fgbi.1997.0988

DO - 10.1006/fgbi.1997.0988

M3 - Article

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AN - SCOPUS:0031168218

SN - 1087-1845

VL - 21

SP - 329

EP - 336

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

IS - 3

ER -

Genome analysis on linkage group VI of Neurospora crassa

Abstract

Keywords

ASJC Scopus subject areas

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