Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis

David E. Greenberg, Stephen F. Porcella, Adrian M. Zelazny, Kimmo Virtaneva, Dan E. Sturdevant, John J. Kupko, Kent D. Barbian, Amenah Babar, David W. Dorward, Steven M. Holland

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Chronic granulomatous disease (CGD) is an inherited immune deficiency characterized by increased susceptibility to infection with Staphylococcus, certain gram-negative bacteria, and fungi. Granulibacter bethesdensis, a newly described genus and species within the family Acetobacteraceae, was recently isolated from four CGD patients residing in geographically distinct locales who presented with fever and lymphadenitis. We sequenced the genome of the reference strain of Granulibacter bethesdensis, which was isolated from lymph nodes of the original patient. The genome contains 2,708,355 base pairs in a single circular chromosome, in which 2,437 putative open reading frames (ORFs) were identified, 1,470 of which share sequence similarity with ORFs in the nonpathogenic but related Gluconobacter oxydans genome. Included in the 967 ORFs that are unique to G. bethesdensis are ORFs potentially important for virulence, adherence, DNA uptake, and methanol utilization. GC% values and best BLAST analysis suggested that some of these unique ORFs were recently acquired. Comparison of G. bethesdensis to other known CGD pathogens demonstrated conservation of some putative virulence factors, suggesting possible common mechanisms involved in pathogenesis in CGD. Genotyping of the four patient isolates by use of a custom microarray demonstrated genome-wide variations in regions encoding DNA uptake systems and transcriptional regulators and in hypothetical ORFs. G. bethesdensis is a genetically diverse emerging human pathogen that may have recently acquired virulence factors new to this family of organisms.

Original languageEnglish (US)
Pages (from-to)8727-8736
Number of pages10
JournalJournal of Bacteriology
Volume189
Issue number23
DOIs
StatePublished - Dec 2007

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Acetic Acid
Open Reading Frames
Sequence Analysis
Chronic Granulomatous Disease
Genome
Bacteria
Virulence Factors
Acetobacteraceae
Gluconobacter oxydans
Lymphadenitis
DNA
Gram-Negative Bacteria
Staphylococcus
Base Pairing
Methanol
Virulence
Fungi
Fever
Chromosomes
Lymph Nodes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Greenberg, D. E., Porcella, S. F., Zelazny, A. M., Virtaneva, K., Sturdevant, D. E., Kupko, J. J., ... Holland, S. M. (2007). Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis. Journal of Bacteriology, 189(23), 8727-8736. https://doi.org/10.1128/JB.00793-07

Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis. / Greenberg, David E.; Porcella, Stephen F.; Zelazny, Adrian M.; Virtaneva, Kimmo; Sturdevant, Dan E.; Kupko, John J.; Barbian, Kent D.; Babar, Amenah; Dorward, David W.; Holland, Steven M.

In: Journal of Bacteriology, Vol. 189, No. 23, 12.2007, p. 8727-8736.

Research output: Contribution to journalArticle

Greenberg, DE, Porcella, SF, Zelazny, AM, Virtaneva, K, Sturdevant, DE, Kupko, JJ, Barbian, KD, Babar, A, Dorward, DW & Holland, SM 2007, 'Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis', Journal of Bacteriology, vol. 189, no. 23, pp. 8727-8736. https://doi.org/10.1128/JB.00793-07
Greenberg, David E. ; Porcella, Stephen F. ; Zelazny, Adrian M. ; Virtaneva, Kimmo ; Sturdevant, Dan E. ; Kupko, John J. ; Barbian, Kent D. ; Babar, Amenah ; Dorward, David W. ; Holland, Steven M. / Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis. In: Journal of Bacteriology. 2007 ; Vol. 189, No. 23. pp. 8727-8736.
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