Genome-wide analysis of KAP1, the 7SK snRNP complex, and RNA polymerase II

Ryan P. McNamara, Carlos Guzman, Jonathan E. Reeder, Iván D'Orso

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The transition of RNA polymerase II (Pol II) from transcription initiation into productive elongation in eukaryotic cells is regulated by the P-TEFb kinase, which phosphorylates the C-terminal domain of paused Pol II at promoter-proximal regions. Our recent study found that P-TEFb (in an inhibited state bound to the 7SK snRNP complex) interacts with the KAP1/TRIM28 transcriptional regulator, and that KAP1 and the 7SK snRNP co-occupy most gene promoters containing paused Pol II. Here we provide a detailed experimental description and analysis of the ChIP-seq datasets that have been deposited into Gene Expression Omnibus (GEO): GS72622, so that independent groups can replicate and expand upon these findings. We propose these datasets would provide valuable information for researchers studying mechanisms of transcriptional regulation including Pol II pausing and pause release.

Original languageEnglish (US)
Pages (from-to)250-255
Number of pages6
JournalGenomics Data
Volume7
DOIs
Publication statusPublished - Mar 1 2016

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Keywords

  • ChIP-seq
  • KAP1
  • P-TEFb/7SK snRNP
  • RNA polymerase II
  • Transcription elongation

ASJC Scopus subject areas

  • Molecular Medicine
  • Biochemistry
  • Biotechnology
  • Genetics

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