Genomic analysis of smooth muscle cells in 3-dimensional collagen matrix.

Song Li, Jianmin Lao, Benjamin P C Chen, Yi shuan Li, Yihua Zhao, Julia Chu, Kuang Den Chen, Tsui Chun Tsou, Konan Peck, Shu Chien

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

The proliferation, differentiation, and protein synthesis of vascular smooth muscle cells (SMCs) play important roles in vascular remodeling. Here, we compared the genetic programming and signaling of SMCs in collagen matrix as a three-dimensional (3-D) environment and on a two-dimensional (2-D) surface. By using DNA microarrays with 9600 genes, we showed that 77 genes were expressed more than twofold and 22 genes were less than one-half in 3-D matrix, when compared with the 2-D condition. The higher expression level of cyclin-dependent kinase inhibitor 1 (p21) in 3-D matrix suggests that p21 may be responsible for the lower proliferation rate in 3-D matrix. The expression level of collagen I was higher in 3-D matrix, suggesting that SMCs in 3-D matrix have increased matrix synthesis. In addition, SMCs in 3-D matrix had less stress fibers and focal adhesions, and a lower level of tyrosine phosphorylation of focal adhesion kinase (FAK). Overexpression of FAK attenuated the expression of p21 and collagen I in 3-D matrix, suggesting that FAK functions as a molecular switch for cell cycle regulation and matrix synthesis. The information generated in this study helps to elucidate the molecular basis of the modulation of SMC phenotypes by the extracellular matrix.

Original languageEnglish (US)
Pages (from-to)97-99
Number of pages3
JournalThe FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume17
Issue number1
StatePublished - 2003

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Smooth Muscle Myocytes
Muscle
Collagen
Focal Adhesion Protein-Tyrosine Kinases
Cells
CDC2 Protein Kinase
Cyclin-Dependent Kinase Inhibitor p21
Genes
Stress Fibers
Focal Adhesions
Oligonucleotide Array Sequence Analysis
Vascular Smooth Muscle
Extracellular Matrix
Tyrosine
Cell Cycle
Phosphorylation
Phenotype
Cyclin-Dependent Kinases
Genetic programming
Microarrays

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Genomic analysis of smooth muscle cells in 3-dimensional collagen matrix. / Li, Song; Lao, Jianmin; Chen, Benjamin P C; Li, Yi shuan; Zhao, Yihua; Chu, Julia; Chen, Kuang Den; Tsou, Tsui Chun; Peck, Konan; Chien, Shu.

In: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 17, No. 1, 2003, p. 97-99.

Research output: Contribution to journalArticle

Li, Song ; Lao, Jianmin ; Chen, Benjamin P C ; Li, Yi shuan ; Zhao, Yihua ; Chu, Julia ; Chen, Kuang Den ; Tsou, Tsui Chun ; Peck, Konan ; Chien, Shu. / Genomic analysis of smooth muscle cells in 3-dimensional collagen matrix. In: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2003 ; Vol. 17, No. 1. pp. 97-99.
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