TY - JOUR
T1 - Genomic and expression analysis of canine calcitonin receptor-stimulating peptides and calcitonin/calcitonin gene-related peptide
AU - Osaki, Tsukasa
AU - Katafuchi, Takeshi
AU - Minamino, Naoto
N1 - Funding Information:
This work was supported in part by Research Grants from the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (NIBIO), and the Ministry of Health, Labor and Welfare (Cardiovascular Diseases, 20C-3) of Japan. We are grateful to Dr K. Kangawa of this institute for peptide synthesis and discussions, Dr H. Yasue of the National Institute of Agrobiological Sciences for discussions and Dr Y. Hayashi of Asubio Pharma Co., Ltd for experimental advice and discussions. We also thank Drs K. Uemura and M. Sugimachi of the Department of Cardiovascular Dynamics, Dr T. Mizuno of the Department of Artificial Organs of this institute for instructions in canine tissue anatomy and dissection, and Ms M. Nakatani and R. Osaki of the Department of Pharmacology of this institute for technical assistance.
PY - 2008/10
Y1 - 2008/10
N2 - Calcitonin receptor-stimulating peptides (CRSPs) are new members of the calcitonin/calcitonin gene-related peptide (CT/CGRP) family identified in pigs, dogs and other domestic animals, and CRSP-1 is an active ligand for the CT receptor (CT-R). We recently sequenced porcine CRSP genes (Crsps) and found similarity with the CT/CGRP gene (Ct/Cgrp) in sequence and genomic organization. In this study, we identified five Crsps, Crsp-1 to Crsp-5, in dogs. Crsp-1 has five exons with an exon-intron organization identical to that of porcine Crsp-1 or Crsp-2, while Crsp-2 and Crsp-3 have additional CT-2- and CT-3-coding exons like Ct/Cgrp. Crsp-2 was renamed as Ct-2/Crsp-2 because both CRSP-2 and CT-2 mRNAs were tissue-specifically expressed. Crsp-4 and Crsp-5 are presumably generated by retrotransposition. We postulate that Crsps were generated from the gene duplication of Ct/Cgrp, and gained their diversity during mammalian evolution. Among the canine CTs and CRSPs, CRSP-1, CT-1 and CT-2 are active ligands for the CT-R, but CRSP-2 and others are inactive. Canine CRSP-1 and CT-2 are expressed in the central and peripheral systems, while CT-1 is localized in the thyroid gland. These findings indicate that dogs can be used for an experimental model as analysing the physiological roles of the CT/CGRP/CRSP family.
AB - Calcitonin receptor-stimulating peptides (CRSPs) are new members of the calcitonin/calcitonin gene-related peptide (CT/CGRP) family identified in pigs, dogs and other domestic animals, and CRSP-1 is an active ligand for the CT receptor (CT-R). We recently sequenced porcine CRSP genes (Crsps) and found similarity with the CT/CGRP gene (Ct/Cgrp) in sequence and genomic organization. In this study, we identified five Crsps, Crsp-1 to Crsp-5, in dogs. Crsp-1 has five exons with an exon-intron organization identical to that of porcine Crsp-1 or Crsp-2, while Crsp-2 and Crsp-3 have additional CT-2- and CT-3-coding exons like Ct/Cgrp. Crsp-2 was renamed as Ct-2/Crsp-2 because both CRSP-2 and CT-2 mRNAs were tissue-specifically expressed. Crsp-4 and Crsp-5 are presumably generated by retrotransposition. We postulate that Crsps were generated from the gene duplication of Ct/Cgrp, and gained their diversity during mammalian evolution. Among the canine CTs and CRSPs, CRSP-1, CT-1 and CT-2 are active ligands for the CT-R, but CRSP-2 and others are inactive. Canine CRSP-1 and CT-2 are expressed in the central and peripheral systems, while CT-1 is localized in the thyroid gland. These findings indicate that dogs can be used for an experimental model as analysing the physiological roles of the CT/CGRP/CRSP family.
KW - Calcitonin
KW - Calcitonin gene-related peptide
KW - Calcitonin receptor
KW - Calcitonin receptor-stimulating peptide
KW - Genomic organization
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U2 - 10.1093/jb/mvn084
DO - 10.1093/jb/mvn084
M3 - Article
C2 - 18558619
AN - SCOPUS:53549108852
SN - 0021-924X
VL - 144
SP - 419
EP - 430
JO - Journal of Biochemistry
JF - Journal of Biochemistry
IS - 4
ER -