Genomic and gene expression profiling of minute alterations of chromosome arm 1p in small-cell lung carcinoma cells

L. J. Henderson, B. P. Coe, E. H L Lee, L. Girard, A. F. Gazdar, J. D. Minna, S. Lam, C. MacAulay, W. L. Lam

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Genetic alterations occurring on human chromosome arm 1p are common in many types of cancer including lung, breast, neuroblastoma, pheochromocytoma, and colorectal. The identification of tumour suppressors and oncogenes on this arm has been limited by the low resolution of current technologies for fine mapping. In order to identify genetic alterations on 1p in small-cell lung carcinoma, we developed a new resource for fine mapping segmental DNA copy number alterations. We have constructed an array of 642 ordered and fingerprint-verified bacterial artificial chromosome clones spanning the 120 megabase (Mb) 1p arm from 1p11.2 to p36.33. The 1p arm of 15 small-cell lung cancer cell lines was analysed at sub-Mb resolution using this arm-specific array. Among the genetic alterations identified, two regions of recurrent amplification emerged. They were detected in at least 45% of the samples: a 580kb region at 1p34.2-p34.3 and a 270kb region at 1p11.2. We further defined the potential importance of these genomic amplifications by analysing the RNA expression of the genes in these regions with Affymetrix oligonucleotide arrays and semiquantitative reverse transcriptase-polymerase chain reaction. Our data revealed overexpression of the genes HEYL, HPCAL4, BMP8, IPT, and RLF, coinciding with genomic amplification.

Original languageEnglish (US)
Pages (from-to)1553-1560
Number of pages8
JournalBritish Journal of Cancer
Volume92
Issue number8
DOIs
StatePublished - Apr 25 2005

Keywords

  • 1p
  • Array CGH
  • Gene expression
  • Small-cell lung carcinoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Genomic and gene expression profiling of minute alterations of chromosome arm 1p in small-cell lung carcinoma cells'. Together they form a unique fingerprint.

  • Cite this