TY - JOUR
T1 - Genomic expression analysis by single-cell mRNA differential display of quiescent CD8 T cells from tumour-infiltrating lymphocytes obtained from in vivo liver tumours
AU - Zhang, Wei
AU - Ding, Jianqing
AU - Qu, Yan
AU - Hu, Hongliang
AU - Lin, Meihua
AU - Datta, Amit
AU - Larson, Alan
AU - Liu, George E.
AU - Li, Biaoru
PY - 2009/5
Y1 - 2009/5
N2 - We performed a genomic study combining single-cell mRNA differential display and RNA subtractive hybridization to elucidate CD8 T-cell quiescence/ignorance. By comparing actively maintained quiescent CD8 T cells from liver tumour tumour-infiltrating lymphocytes (TILs) with quiescent T cells at the single-cell level, we identified differentially expressed candidate genes by high-throughput screening and comparative analysis of expressed sequence tags (ESTs). While genes for the T-cell receptor, tumour necrosis factor (TNF) receptor, TNF-related apoptpsis inducing ligand (TRAIL) and perforin were down-regulated, key genes such as Tob, transforming growth factor (TGF)-β, lung Krüpple-like factor (LKLF), Sno-A, Ski, Myc, Ets-2 repressor factor (ERF) and RE1-silencing transcription factor (REST/NRSF) complex were highly expressed in the quiescent TIL CD8 cells. Real-time polymerase chain reaction (PCR) further confirmed these results. A regulation model is proposed for actively maintained quiescence in CD8 T cells, including three components: up-regulation of the TGF-β pathway, a shift in the MYC web and inhibition of the cell cycle.
AB - We performed a genomic study combining single-cell mRNA differential display and RNA subtractive hybridization to elucidate CD8 T-cell quiescence/ignorance. By comparing actively maintained quiescent CD8 T cells from liver tumour tumour-infiltrating lymphocytes (TILs) with quiescent T cells at the single-cell level, we identified differentially expressed candidate genes by high-throughput screening and comparative analysis of expressed sequence tags (ESTs). While genes for the T-cell receptor, tumour necrosis factor (TNF) receptor, TNF-related apoptpsis inducing ligand (TRAIL) and perforin were down-regulated, key genes such as Tob, transforming growth factor (TGF)-β, lung Krüpple-like factor (LKLF), Sno-A, Ski, Myc, Ets-2 repressor factor (ERF) and RE1-silencing transcription factor (REST/NRSF) complex were highly expressed in the quiescent TIL CD8 cells. Real-time polymerase chain reaction (PCR) further confirmed these results. A regulation model is proposed for actively maintained quiescence in CD8 T cells, including three components: up-regulation of the TGF-β pathway, a shift in the MYC web and inhibition of the cell cycle.
KW - CD8
KW - Gene expression profiling
KW - Quiescence
KW - T cells
KW - Tumour-infiltrating lymphocytes (TILs)
UR - http://www.scopus.com/inward/record.url?scp=63849221409&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=63849221409&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2567.2008.02926.x
DO - 10.1111/j.1365-2567.2008.02926.x
M3 - Article
C2 - 18778280
AN - SCOPUS:63849221409
SN - 0019-2805
VL - 127
SP - 83
EP - 90
JO - Immunology
JF - Immunology
IS - 1
ER -