Genomic profiling of neutrophil transcripts in Asian Qigong practitioners: A pilot study in gene regulation by mind-body interaction

Quan Zhen Li, Ping Li, Gabriela E. Garcia, Richard J. Johnson, Lili Feng

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background and objectives: The great similarity of the genomes of humans and other species stimulated us to search for genes regulated by elements associated with human uniqueness, such as the mind-body interaction. DNA microarray technology offers the advantage of analyzing thousands of genes simultaneously, with the potential to determine healthy phenotypic changes in gene expression. The aim of this study was to determine the genomic profile and function of neutrophils in Falun Gong (FLG, an ancient Chinese Qigong) practitioners, with healthy subjects as controls. Subjects and design: Six (6) Asian FLG practitioners and 6 Asian normal healthy controls were recruited for our study. The practitioners have practiced FLG for at least 1 year (range, 1-5 years). The practice includes daily reading of FLG books and daily practice of exercises lasting 1-2 hours. Selected normal healthy controls did not perform Qigong, yoga, t'ai chi, or any other type of mind-body practice, and had not followed any conventional physical exercise program for at least 1 year. Neutrophils were isolated from fresh blood and assayed for gene expression, using microarrays and RNase protection assay (RPA), as well as for function (phagocytosis) and survival (apoptosis). Results: The changes in gene expression of FLG practitioners in contrast to normal healthy controls were characterized by enhanced immunity, downregulation of cellular metabolism, and alteration of apoptotic genes in favor of a rapid resolution of inflammation. The lifespan of normal neutrophils was prolonged, while the inflammatory neutrophils displayed accelerated cell death in FLG practitioners as determined by enzyme-linked immunosorbent assay. Correlating with enhanced immunity reflected by microarray data, neutrophil phagocytosis was significantly increased in Qigong practitioners. Some of the altered genes observed by microarray were confirmed by RPA. Conclusion: Qigong practice may regulate immunity, metabolic rate, and cell death, possibly at the transcriptional level. Our pilot study provides the first evidence that Qigong practice may exert transcriptional regulation at a genomic level. New approaches are needed to study how genes are regulated by elements associated with human uniqueness, such as consciousness, cognition, and spirituality.

Original languageEnglish (US)
Pages (from-to)29-39
Number of pages11
JournalJournal of Alternative and Complementary Medicine
Volume11
Issue number1
DOIs
StatePublished - Feb 2005

Fingerprint

Qigong
Neutrophils
Genes
Ribonucleases
Phagocytosis
Gene Expression
Immunity
Cell Death
Exercise
Yoga
Somatotypes
Spirituality
Human Genome
Oligonucleotide Array Sequence Analysis
Consciousness
Cellular Immunity
Cognition
Reading
Healthy Volunteers
Down-Regulation

ASJC Scopus subject areas

  • Medicine(all)
  • Complementary and alternative medicine
  • Nursing(all)

Cite this

Genomic profiling of neutrophil transcripts in Asian Qigong practitioners : A pilot study in gene regulation by mind-body interaction. / Li, Quan Zhen; Li, Ping; Garcia, Gabriela E.; Johnson, Richard J.; Feng, Lili.

In: Journal of Alternative and Complementary Medicine, Vol. 11, No. 1, 02.2005, p. 29-39.

Research output: Contribution to journalArticle

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