Genomic structure and chromosomal assignment of the mouse Ku70 gene

Yuichi Takiguchi, Akihiro Kurimasa, Fanqing Chen, Paige E. Pardington, Takayuki Kuriyama, Richard T. Okinaka, Robert Moyzis, David J. Chen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

DNA-dependent protein kinase (DNA-PK) consists of three polypeptide subunits: Ku70, Ku80, and the DNA-PK catalytic subunit (DNA-PKcs). Mammalian mutants deficient in either Ku80 or DNA-PKcs function have been shown to be lacking in DNA double-strand break repair and V(D)J recombination, respectively. The precise role of the Ku70 gene in this process has not yet been determined, in part because no cell lines, animals, or human diseases involved with deficiencies in this gene have yet been identified. Both the human and the mouse Ku70 cDNAs have been cloned, and the human gene has been mapped to chromosome 22q13. The original mouse cDNA clones, however, lacked a complete 5'-region, and none of the mammalian Ku70 genomic sequences have been characterized. This report contains an analysis of the 5'-region of the mouse cDNA sequence, a characterization of the mouse Ku70 genomic structure, and fluorescence in situ hybridization data that map the mouse gene to chromosome 15. The deduced amino acid sequence of the mouse gene consists of 608 amino acids compared to 609 for the human gene. The genomic sequence is 24 kb and consists of 13 exons, including an untranslated first exon. Sequences from the upstream region of exon 1 revealed four consensus GC box sequences and a strong transcription initiation site at a reasonable location. The assignment of the mouse Ku70 gene to chromosome 15 is consistent with the syntenic relationship of this gene in human (chromosome 22q13) and mouse and adds to the comparative mapping data for the genes involved in the SCID phenotype.

Original languageEnglish (US)
Pages (from-to)129-135
Number of pages7
JournalGenomics
Volume35
Issue number1
DOIs
StatePublished - Jul 1 1996

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Genes
DNA-Activated Protein Kinase
Chromosomes, Human, Pair 15
Exons
Complementary DNA
Catalytic Domain
V(D)J Recombination
Double-Stranded DNA Breaks
Chromosome Mapping
Transcription Initiation Site
Human Chromosomes
Fluorescence In Situ Hybridization
Amino Acid Sequence
Clone Cells
Chromosomes
Phenotype
Amino Acids
Cell Line
Peptides
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Takiguchi, Y., Kurimasa, A., Chen, F., Pardington, P. E., Kuriyama, T., Okinaka, R. T., ... Chen, D. J. (1996). Genomic structure and chromosomal assignment of the mouse Ku70 gene. Genomics, 35(1), 129-135. https://doi.org/10.1006/geno.1996.0331

Genomic structure and chromosomal assignment of the mouse Ku70 gene. / Takiguchi, Yuichi; Kurimasa, Akihiro; Chen, Fanqing; Pardington, Paige E.; Kuriyama, Takayuki; Okinaka, Richard T.; Moyzis, Robert; Chen, David J.

In: Genomics, Vol. 35, No. 1, 01.07.1996, p. 129-135.

Research output: Contribution to journalArticle

Takiguchi, Y, Kurimasa, A, Chen, F, Pardington, PE, Kuriyama, T, Okinaka, RT, Moyzis, R & Chen, DJ 1996, 'Genomic structure and chromosomal assignment of the mouse Ku70 gene', Genomics, vol. 35, no. 1, pp. 129-135. https://doi.org/10.1006/geno.1996.0331
Takiguchi Y, Kurimasa A, Chen F, Pardington PE, Kuriyama T, Okinaka RT et al. Genomic structure and chromosomal assignment of the mouse Ku70 gene. Genomics. 1996 Jul 1;35(1):129-135. https://doi.org/10.1006/geno.1996.0331
Takiguchi, Yuichi ; Kurimasa, Akihiro ; Chen, Fanqing ; Pardington, Paige E. ; Kuriyama, Takayuki ; Okinaka, Richard T. ; Moyzis, Robert ; Chen, David J. / Genomic structure and chromosomal assignment of the mouse Ku70 gene. In: Genomics. 1996 ; Vol. 35, No. 1. pp. 129-135.
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