Genomic structure of the mouse apurinic/apyrimidinic endonuclease gene

Y. Takiguchi, D. J. Chen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A mammalian apurinic/apyrimidinic endonuclease (AP endonuclease) is known to have two distinct functional domains. One domain is responsible for regulating the activity of Fos/Jun proto-oncogene products to bind to DNA at specific recognition sites. The other domain which is highly conserved from bacteria to mammals, is responsible for repairing DNA damage caused by ionizing radiation, oxidative damage, and alkylating agents. This study reports on the isolation and characterization of the genomic structure of the mouse AP endonuclease gene (Apex). The genomic sequence of the Apex gene was 2.14 kb in length and contained four exons. Exon 1 contained a 0.24-kb untranslated 5′ region upstream of the initiation codon. Consensus sequences for two CAAT boxes and a GC box were found upstream of the end of exon 1. A polymorphism was noted in the untranslated region of exon 1 in a comparison of a number of mouse strains. These data indicate that the 5′ end of the mouse gene (Apex) differs from the previously isolated human gene (Ape), which has five exons and an untranslated region between exons 1 and 2. Data are also presented that suggest the presence of two pseudogenes in the mouse.

Original languageEnglish (US)
Pages (from-to)717-722
Number of pages6
JournalMammalian Genome
Volume5
Issue number11
DOIs
StatePublished - Nov 1994

Fingerprint

DNA-(Apurinic or Apyrimidinic Site) Lyase
Exons
Genes
Untranslated Regions
jun Genes
Pseudogenes
Initiator Codon
5' Untranslated Regions
Endonucleases
Alkylating Agents
Oncogene Proteins
Hominidae
Consensus Sequence
Ionizing Radiation
DNA Damage
Mammals
Bacteria
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Genomic structure of the mouse apurinic/apyrimidinic endonuclease gene. / Takiguchi, Y.; Chen, D. J.

In: Mammalian Genome, Vol. 5, No. 11, 11.1994, p. 717-722.

Research output: Contribution to journalArticle

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