Genomics in premature infants

A non-invasive strategy to obtain high-quality DNA

Mariam Said, Clint Cappiello, Joseph M. Devaney, Daniele Podini, Alana L. Beres, Stanislav Vukmanovic, Khodayar Rais-Bahrami, Naomi C. Luban, Anthony D. Sandler, Zohreh Tatari-Calderone

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We used a cost-effective, non-invasive method to obtain high-quality DNA from buccal epithelial-cells (BEC) of premature infants for genomic analysis. DNAs from BEC were obtained from premature infants with gestational age ≤ 36 weeks. Short terminal repeats (STRs) were performed simultaneously on DNA obtained from the buccal swabs and blood from the same patient. The STR profiles demonstrated that the samples originated from the same individual and exclude any contamination by external DNAs. Whole exome sequencing was performed on DNAs obtained from BEC on premature infants with and without necrotizing enterocolitis, and successfully provided a total number of reads and variants corroborating with those obtained from healthy blood donors. We provide a proof of concept that BEC is a reliable and preferable source of DNA for high-throughput sequencing in premature infants.

Original languageEnglish (US)
Article number4286
JournalScientific Reports
Volume4
DOIs
StatePublished - Mar 6 2014

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Genomics
Premature Infants
Epithelial Cells
DNA
Terminal Repeat Sequences
DNA Contamination
Exome
High-Throughput Nucleotide Sequencing
Necrotizing Enterocolitis
Blood Donors
Gestational Age
Costs and Cost Analysis

ASJC Scopus subject areas

  • General

Cite this

Said, M., Cappiello, C., Devaney, J. M., Podini, D., Beres, A. L., Vukmanovic, S., ... Tatari-Calderone, Z. (2014). Genomics in premature infants: A non-invasive strategy to obtain high-quality DNA. Scientific Reports, 4, [4286]. https://doi.org/10.1038/srep04286

Genomics in premature infants : A non-invasive strategy to obtain high-quality DNA. / Said, Mariam; Cappiello, Clint; Devaney, Joseph M.; Podini, Daniele; Beres, Alana L.; Vukmanovic, Stanislav; Rais-Bahrami, Khodayar; Luban, Naomi C.; Sandler, Anthony D.; Tatari-Calderone, Zohreh.

In: Scientific Reports, Vol. 4, 4286, 06.03.2014.

Research output: Contribution to journalArticle

Said, M, Cappiello, C, Devaney, JM, Podini, D, Beres, AL, Vukmanovic, S, Rais-Bahrami, K, Luban, NC, Sandler, AD & Tatari-Calderone, Z 2014, 'Genomics in premature infants: A non-invasive strategy to obtain high-quality DNA', Scientific Reports, vol. 4, 4286. https://doi.org/10.1038/srep04286
Said, Mariam ; Cappiello, Clint ; Devaney, Joseph M. ; Podini, Daniele ; Beres, Alana L. ; Vukmanovic, Stanislav ; Rais-Bahrami, Khodayar ; Luban, Naomi C. ; Sandler, Anthony D. ; Tatari-Calderone, Zohreh. / Genomics in premature infants : A non-invasive strategy to obtain high-quality DNA. In: Scientific Reports. 2014 ; Vol. 4.
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