Germ cell tumor cell culture techniques

John T. Lafin, James F. Amatruda, Aditya Bagrodia

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Optimization of cell culture protocol for a given cell line is critical for the proper conduct of in vitro experiments. Because germ cell tumors can be so heterogeneous, optimal culture conditions can vary widely between cell lines. Here, we describe our experience in routine culture and cryopreservation of germ cell tumor cell culture. Additionally, methods for measuring cell viability and proliferation validated on these lines are provided.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages65-76
Number of pages12
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2195
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cell culture
  • Embryonal carcinoma
  • Germ cell tumor
  • Seminoma
  • Yolk sac tumor

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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