Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells

B. R. Stanton; S. W. Reid; L. F. Parada

doi:10.1128/MCB.10.12.6755

Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells

B. R. Stanton, S. W. Reid, L. F. Parada

Developmental Biology

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

We have disrupted one allele of the N-myc locus in mouse embryonic stem (ES) cells by using homologous recombination techniques and have obtained germ line transmission of null N-myc ES cell lines with transmission of the null N-myc allele to the offspring. The creation of mice with a deficient N-myc allele will allow the generation of offspring bearing null N-myc alleles in both chromosomes and permit study of the role that this proto-oncogene plays in embryonic development.

Original language	English (US)
Pages (from-to)	6755-6758
Number of pages	4
Journal	Molecular and cellular biology
Volume	10
Issue number	12
DOIs	https://doi.org/10.1128/MCB.10.12.6755
State	Published - 1990

ASJC Scopus subject areas

Molecular Biology
Cell Biology

Access to Document

10.1128/MCB.10.12.6755

Cite this

@article{9563b355d67d4df59607e8ee93c0a81d,

title = "Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells",

abstract = "We have disrupted one allele of the N-myc locus in mouse embryonic stem (ES) cells by using homologous recombination techniques and have obtained germ line transmission of null N-myc ES cell lines with transmission of the null N-myc allele to the offspring. The creation of mice with a deficient N-myc allele will allow the generation of offspring bearing null N-myc alleles in both chromosomes and permit study of the role that this proto-oncogene plays in embryonic development.",

author = "Stanton, {B. R.} and Reid, {S. W.} and Parada, {L. F.}",

year = "1990",

doi = "10.1128/MCB.10.12.6755",

language = "English (US)",

volume = "10",

pages = "6755--6758",

journal = "Molecular and cellular biology",

issn = "0270-7306",

publisher = "American Society for Microbiology",

number = "12",

}

TY - JOUR

T1 - Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells

AU - Stanton, B. R.

AU - Reid, S. W.

AU - Parada, L. F.

PY - 1990

Y1 - 1990

N2 - We have disrupted one allele of the N-myc locus in mouse embryonic stem (ES) cells by using homologous recombination techniques and have obtained germ line transmission of null N-myc ES cell lines with transmission of the null N-myc allele to the offspring. The creation of mice with a deficient N-myc allele will allow the generation of offspring bearing null N-myc alleles in both chromosomes and permit study of the role that this proto-oncogene plays in embryonic development.

AB - We have disrupted one allele of the N-myc locus in mouse embryonic stem (ES) cells by using homologous recombination techniques and have obtained germ line transmission of null N-myc ES cell lines with transmission of the null N-myc allele to the offspring. The creation of mice with a deficient N-myc allele will allow the generation of offspring bearing null N-myc alleles in both chromosomes and permit study of the role that this proto-oncogene plays in embryonic development.

UR - http://www.scopus.com/inward/record.url?scp=0025258855&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025258855&partnerID=8YFLogxK

U2 - 10.1128/MCB.10.12.6755

DO - 10.1128/MCB.10.12.6755

M3 - Article

C2 - 1701023

AN - SCOPUS:0025258855

SN - 0270-7306

VL - 10

SP - 6755

EP - 6758

JO - Molecular and cellular biology

JF - Molecular and cellular biology

IS - 12

ER -

Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this