Gilgamesh (Gish)/CK1γ regulates tissue homeostasis and aging in adult drosophila midgut

Shuangxi Li, Aiguo Tian, Shuang Li, Yuhong Han, Bing Wang, Jin Jiang

Research output: Contribution to journalArticlepeer-review

Abstract

Adult tissues and organs rely on resident stem cells to generate new cells that replenish damaged cells. To maintain homeostasis, stem cell activity needs to be tightly controlled throughout the adult life. Here, we show that the membrane-associated kinase Gilgamesh (Gish)/CK1γ maintains Drosophila adult midgut homeostasis by restricting JNK pathway activity and that Gish is essential for intestinal stem cell (ISC) maintenance under stress conditions. Inactivation of Gish resulted in aberrant JNK pathway activation and excessive production of multiple cytokines and growth factors that drive ISC overproliferation. Mechanistically, Gish restricts JNK activation by phosphorylating and destabilizing a small GTPase, Rho1. Interestingly, we find that Gish expression is down-regulated in aging guts and that increasing Gish activity in aging guts can restore tissue homeostasis. Hence, our study identifies Gish/CK1γ as a novel regulator of Rho1 and gatekeeper of tissue homeostasis whose activity is compromised in aging guts.

Original languageEnglish (US)
Article numbere201909103
JournalJournal of Cell Biology
Volume219
Issue number4
DOIs
StatePublished - 2020

ASJC Scopus subject areas

  • Cell Biology

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