Glia-and neuron-specific functions of TrkB signalling during retinal degeneration and regeneration

Chikako Harada, Xiaoli Guo, Kazuhiko Namekata, Atsuko Kimura, Kazuaki Nakamura, Kohichi Tanaka, Luis F. Parada, Takayuki Harada

Research output: Contribution to journalArticle

68 Scopus citations

Abstract

Glia, the support cells of the central nervous system, have recently attracted considerable attention both as mediators of neural cell survival and as sources of neural regeneration. To further elucidate the role of glial and neural cells in neurodegeneration, we generated TrkBGFAP and TrkB c-kit knockout mice in which TrkB, a receptor for brain-derived neurotrophic factor (BDNF), is deleted in retinal glia or inner retinal neurons, respectively. Here, we show that the extent of glutamate-induced retinal degeneration was similar in these two mutant mice. Furthermore in TrkB GFAP knockout mice, BDNF did not prevent photoreceptor degeneration and failed to stimulate Müller glial cell proliferation and expression of neural markers in the degenerating retina. These results demonstrate that BDNF signalling in glia has important roles in neural protection and regeneration, particularly in conversion of Müller glia to photoreceptors. In addition, our genetic models provide a system in which glia-and neuron-specific gene functions can be tested in central nervous system tissues in vivo.

Original languageEnglish (US)
Article number189
JournalNature communications
Volume2
Issue number1
DOIs
StatePublished - 2011

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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    Harada, C., Guo, X., Namekata, K., Kimura, A., Nakamura, K., Tanaka, K., Parada, L. F., & Harada, T. (2011). Glia-and neuron-specific functions of TrkB signalling during retinal degeneration and regeneration. Nature communications, 2(1), [189]. https://doi.org/10.1038/ncomms1190