Global analysis of gene expression in cells of the immune system. II. Cell-free translation products and high-density filter hybridization data

Ivan Lefkovits, John R. Kettman, Johann Rudolf Frey

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

We have developed an experimental system for linking information on cell-free transcription and translation products from cDNA clones with data obtained from hybridization signals from complex probes. The work described in this paper consists of two distinct processes, one being the construction of a system of clonal addresses and the other the identification of expressed genes involved in the studied processes. We describe the use of this system to identify genes involved in thymus development. Complex probes from fetal thymuses (GD15, 17 and newborn) of Balb/c mice were used to identify genes, which are up- or downregulated during the process of differentiation. The full set of information is available in the Clone-base of the Basel Institute for Immunology and will be retrievable from the website of the collaborating laboratories.

Original languageEnglish (US)
Pages (from-to)2694-2702
Number of pages9
JournalELECTROPHORESIS
Volume21
Issue number13
DOIs
StatePublished - Jan 1 2000

Keywords

  • Cell-free translation products
  • Gene expression
  • Global protein analysis
  • High-density filter hybridization

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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