Global gene expression analysis of human bronchial epithelial cells treated with tobacco condensates

Ellen D. Jorgensen, Igor Dozmorov, Mark B. Frank, Michael Centola, Anthony P. Albino

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Gene expression patterns were assessed in normal human bronchial epithelial (NHBE) cells exposed to cigarette smoke condensates (CSC) from commercial cigarettes in order to develop a better understanding of the genomic impact of tobacco exposure, and to define biomarkers that can potentially discriminate tobacco-related effects and outcomes in a clinical setting. NHBE cells were treated with CSCs from two American brands for up to 12 hours in the presence of S9 microsomal fraction from Aroclor 1254-treated rats. High-density oligonucleotide microarrays coupled with a novel statistical analysis that relies on statistical significance levels rather than arbitrary fold-change differences was used to identify genes that undergo expression alterations upon treatment. Expression patterns of approximately 3700 genes were altered after CSC treatments. While a majority of these genes were affected by both CSCs, each condensate also affected a unique subset of ∼1000 genes. An unexpected finding was that S9, required for metabolizing procarcinogens in CSCs to carcinogenic metabolites, also altered the expression of approximately 1700 genes. Exposure of NHBE cells to different CSCs alters the expression of a large set of genes that affect a common set of biological pathways including those relevant to carcinogenesis. Identification of CSC-affected genes and underlying biological processes may generate an atlas of molecular events that includes biomarkers of tobacco exposure and disease status in smokers. Finally, the finding that S9 affects the expression of a number of genes may have implications for various toxicogenetic assays currently used by regulatory agencies to evaluate harmful effects in exposed humans.

Original languageEnglish (US)
Pages (from-to)1154-1168
Number of pages15
JournalCell Cycle
Volume3
Issue number9
StatePublished - Sep 2004

Fingerprint

Tobacco
Gene expression
Genes
Epithelial Cells
Gene Expression
Tobacco Products
Smoke
Biomarkers
Condensate treatment
Toxicogenetics
Chlorodiphenyl (54% Chlorine)
Biological Phenomena
Atlases
Oligonucleotide Array Sequence Analysis
Microarrays
Metabolites
Oligonucleotides
Carcinogenesis
Rats
Assays

Keywords

  • Bronchial cell
  • Cigarette smoke
  • Microarray
  • S9 toxicity

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Cite this

Jorgensen, E. D., Dozmorov, I., Frank, M. B., Centola, M., & Albino, A. P. (2004). Global gene expression analysis of human bronchial epithelial cells treated with tobacco condensates. Cell Cycle, 3(9), 1154-1168.

Global gene expression analysis of human bronchial epithelial cells treated with tobacco condensates. / Jorgensen, Ellen D.; Dozmorov, Igor; Frank, Mark B.; Centola, Michael; Albino, Anthony P.

In: Cell Cycle, Vol. 3, No. 9, 09.2004, p. 1154-1168.

Research output: Contribution to journalArticle

Jorgensen, ED, Dozmorov, I, Frank, MB, Centola, M & Albino, AP 2004, 'Global gene expression analysis of human bronchial epithelial cells treated with tobacco condensates', Cell Cycle, vol. 3, no. 9, pp. 1154-1168.
Jorgensen, Ellen D. ; Dozmorov, Igor ; Frank, Mark B. ; Centola, Michael ; Albino, Anthony P. / Global gene expression analysis of human bronchial epithelial cells treated with tobacco condensates. In: Cell Cycle. 2004 ; Vol. 3, No. 9. pp. 1154-1168.
@article{6fb144550ff84b6f9b5a761b39cd2b0e,
title = "Global gene expression analysis of human bronchial epithelial cells treated with tobacco condensates",
abstract = "Gene expression patterns were assessed in normal human bronchial epithelial (NHBE) cells exposed to cigarette smoke condensates (CSC) from commercial cigarettes in order to develop a better understanding of the genomic impact of tobacco exposure, and to define biomarkers that can potentially discriminate tobacco-related effects and outcomes in a clinical setting. NHBE cells were treated with CSCs from two American brands for up to 12 hours in the presence of S9 microsomal fraction from Aroclor 1254-treated rats. High-density oligonucleotide microarrays coupled with a novel statistical analysis that relies on statistical significance levels rather than arbitrary fold-change differences was used to identify genes that undergo expression alterations upon treatment. Expression patterns of approximately 3700 genes were altered after CSC treatments. While a majority of these genes were affected by both CSCs, each condensate also affected a unique subset of ∼1000 genes. An unexpected finding was that S9, required for metabolizing procarcinogens in CSCs to carcinogenic metabolites, also altered the expression of approximately 1700 genes. Exposure of NHBE cells to different CSCs alters the expression of a large set of genes that affect a common set of biological pathways including those relevant to carcinogenesis. Identification of CSC-affected genes and underlying biological processes may generate an atlas of molecular events that includes biomarkers of tobacco exposure and disease status in smokers. Finally, the finding that S9 affects the expression of a number of genes may have implications for various toxicogenetic assays currently used by regulatory agencies to evaluate harmful effects in exposed humans.",
keywords = "Bronchial cell, Cigarette smoke, Microarray, S9 toxicity",
author = "Jorgensen, {Ellen D.} and Igor Dozmorov and Frank, {Mark B.} and Michael Centola and Albino, {Anthony P.}",
year = "2004",
month = "9",
language = "English (US)",
volume = "3",
pages = "1154--1168",
journal = "Cell Cycle",
issn = "1538-4101",
publisher = "Landes Bioscience",
number = "9",

}

TY - JOUR

T1 - Global gene expression analysis of human bronchial epithelial cells treated with tobacco condensates

AU - Jorgensen, Ellen D.

AU - Dozmorov, Igor

AU - Frank, Mark B.

AU - Centola, Michael

AU - Albino, Anthony P.

PY - 2004/9

Y1 - 2004/9

N2 - Gene expression patterns were assessed in normal human bronchial epithelial (NHBE) cells exposed to cigarette smoke condensates (CSC) from commercial cigarettes in order to develop a better understanding of the genomic impact of tobacco exposure, and to define biomarkers that can potentially discriminate tobacco-related effects and outcomes in a clinical setting. NHBE cells were treated with CSCs from two American brands for up to 12 hours in the presence of S9 microsomal fraction from Aroclor 1254-treated rats. High-density oligonucleotide microarrays coupled with a novel statistical analysis that relies on statistical significance levels rather than arbitrary fold-change differences was used to identify genes that undergo expression alterations upon treatment. Expression patterns of approximately 3700 genes were altered after CSC treatments. While a majority of these genes were affected by both CSCs, each condensate also affected a unique subset of ∼1000 genes. An unexpected finding was that S9, required for metabolizing procarcinogens in CSCs to carcinogenic metabolites, also altered the expression of approximately 1700 genes. Exposure of NHBE cells to different CSCs alters the expression of a large set of genes that affect a common set of biological pathways including those relevant to carcinogenesis. Identification of CSC-affected genes and underlying biological processes may generate an atlas of molecular events that includes biomarkers of tobacco exposure and disease status in smokers. Finally, the finding that S9 affects the expression of a number of genes may have implications for various toxicogenetic assays currently used by regulatory agencies to evaluate harmful effects in exposed humans.

AB - Gene expression patterns were assessed in normal human bronchial epithelial (NHBE) cells exposed to cigarette smoke condensates (CSC) from commercial cigarettes in order to develop a better understanding of the genomic impact of tobacco exposure, and to define biomarkers that can potentially discriminate tobacco-related effects and outcomes in a clinical setting. NHBE cells were treated with CSCs from two American brands for up to 12 hours in the presence of S9 microsomal fraction from Aroclor 1254-treated rats. High-density oligonucleotide microarrays coupled with a novel statistical analysis that relies on statistical significance levels rather than arbitrary fold-change differences was used to identify genes that undergo expression alterations upon treatment. Expression patterns of approximately 3700 genes were altered after CSC treatments. While a majority of these genes were affected by both CSCs, each condensate also affected a unique subset of ∼1000 genes. An unexpected finding was that S9, required for metabolizing procarcinogens in CSCs to carcinogenic metabolites, also altered the expression of approximately 1700 genes. Exposure of NHBE cells to different CSCs alters the expression of a large set of genes that affect a common set of biological pathways including those relevant to carcinogenesis. Identification of CSC-affected genes and underlying biological processes may generate an atlas of molecular events that includes biomarkers of tobacco exposure and disease status in smokers. Finally, the finding that S9 affects the expression of a number of genes may have implications for various toxicogenetic assays currently used by regulatory agencies to evaluate harmful effects in exposed humans.

KW - Bronchial cell

KW - Cigarette smoke

KW - Microarray

KW - S9 toxicity

UR - http://www.scopus.com/inward/record.url?scp=13944255788&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13944255788&partnerID=8YFLogxK

M3 - Article

C2 - 15326394

AN - SCOPUS:13944255788

VL - 3

SP - 1154

EP - 1168

JO - Cell Cycle

JF - Cell Cycle

SN - 1538-4101

IS - 9

ER -