Globins Synthesize the Second Messenger Bis-(3′-5′)-Cyclic Diguanosine Monophosphate in Bacteria

Xuehua Wan, Jason R. Tuckerman, Jennifer A. Saito, Tracey Allen K Freitas, James S. Newhouse, Judith R. Denery, Michael Y. Galperin, Gonzalo Gonzalez, Marie Alda Gilles-Gonzalez, Maqsudul Alam

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

Globin-coupled sensors are heme-binding signal transducers in Bacteria and Archaea in which an N-terminal globin controls the activity of a variable C-terminal domain. Here, we report that BpeGReg, a globin-coupled diguanylate cyclase from the whooping cough pathogen Bordetella pertussis, synthesizes the second messenger bis-(3′-5′)-cyclic diguanosine monophosphate (c-di-GMP) upon oxygen binding. Expression of BpeGReg in Salmonella typhimurium enhances biofilm formation, while knockout of the BpeGReg gene of B. pertussis results in decreased biofilm formation. These results represent the first identification a signal ligand for any diguanylate cyclase and provide definitive experimental evidence that a globin-coupled sensor regulates c-di-GMP synthesis and biofilm formation. We propose that the synthesis of c-di-GMP by globin sensors is a widespread phenomenon in bacteria.

Original languageEnglish (US)
Pages (from-to)262-270
Number of pages9
JournalJournal of Molecular Biology
Volume388
Issue number2
DOIs
StatePublished - May 1 2009

Keywords

  • biofilm
  • c-di-GMP
  • diguanylate cyclase
  • globin
  • oxygen sensor

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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    Wan, X., Tuckerman, J. R., Saito, J. A., Freitas, T. A. K., Newhouse, J. S., Denery, J. R., Galperin, M. Y., Gonzalez, G., Gilles-Gonzalez, M. A., & Alam, M. (2009). Globins Synthesize the Second Messenger Bis-(3′-5′)-Cyclic Diguanosine Monophosphate in Bacteria. Journal of Molecular Biology, 388(2), 262-270. https://doi.org/10.1016/j.jmb.2009.03.015