@article{95244fc95457477aa79bd243252030c0,
title = "Globins Synthesize the Second Messenger Bis-(3′-5′)-Cyclic Diguanosine Monophosphate in Bacteria",
abstract = "Globin-coupled sensors are heme-binding signal transducers in Bacteria and Archaea in which an N-terminal globin controls the activity of a variable C-terminal domain. Here, we report that BpeGReg, a globin-coupled diguanylate cyclase from the whooping cough pathogen Bordetella pertussis, synthesizes the second messenger bis-(3′-5′)-cyclic diguanosine monophosphate (c-di-GMP) upon oxygen binding. Expression of BpeGReg in Salmonella typhimurium enhances biofilm formation, while knockout of the BpeGReg gene of B. pertussis results in decreased biofilm formation. These results represent the first identification a signal ligand for any diguanylate cyclase and provide definitive experimental evidence that a globin-coupled sensor regulates c-di-GMP synthesis and biofilm formation. We propose that the synthesis of c-di-GMP by globin sensors is a widespread phenomenon in bacteria.",
keywords = "biofilm, c-di-GMP, diguanylate cyclase, globin, oxygen sensor",
author = "Xuehua Wan and Tuckerman, {Jason R.} and Saito, {Jennifer A.} and Freitas, {Tracey Allen K} and Newhouse, {James S.} and Denery, {Judith R.} and Galperin, {Michael Y.} and Gonzalo Gonzalez and Gilles-Gonzalez, {Marie Alda} and Maqsudul Alam",
note = "Funding Information: This work was supported by the National Science Foundation (grant no. MCB0446431) and the U.S. Army Telemedicine and Advanced Technology Research Center (award no. W81XWH0520013) (M.A.); by the National Institutes of Health Intramural Research Program at the National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health (M.Y.G.); and by the National Science Foundation (grant no. 620531) and Welch Foundation (grant no. I-1575) (M.A.G.G.). We thank Dr. C. Locht for providing the pFUS2 vector; Dr. G. Erdem, J. F. Teiber, and G. L. Kramer for providing laboratory facilities; Dr. D. Raze and C. Mizumoto for technical assistance; Dr. H. Wahab and N. Bahiyahn for initial homology modeling; Dr. A. C. Whelen for providing the clinical strain of B. pertussis ; Dr. E. H. S. Sousa for discussions; and Dr. G. Hazelbauer, Dr. C. Appleby, Dr. P. Patek, and Dr. D. Hunt for critical reading of the manuscript. ",
year = "2009",
month = may,
day = "1",
doi = "10.1016/j.jmb.2009.03.015",
language = "English (US)",
volume = "388",
pages = "262--270",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "2",
}